Simultaneous existence of cinnamomin (a type II RIP) and small amount of its free A- and B-chain in mature seeds of camphor tree
文献类型：期刊论文
| 作者 | Hou, FJ; Xu, H; Liu, WY |
| 刊名 | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:4页码:455-464 |
| 关键词 | cinnamomin disultide bond-cleaving enzymes free A- and B-chain type II RIP |
| 通讯作者 | Liu, WY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Cinnamomin, a type 11 ribosome-inactivating protein (RIP), was isolated from the mature seeds of camphor tree (Cinnamomum camphora). In this paper, small amount of free A- and B-chain of cinnamomin were found to be present in the mature seed cell of C. camphora besides the intact cinnamomin. Our results demonstrated that camphorin, a type I RIP previously reported to coexist with cinnamomin in the seeds of C camphora, actually was the A-chain of cinnamomin. The percentage of free A- and B-chain in the total cinnamomin was 2.6-2.8% in the seed extract. Of these free A- and B-chain approximate 80% already existed in the seed cell, only about 20% were produced during the purification operation. As the enzymatic activity to reduce disulfide bond of cinnamomin in the seed extract of C camphora was detected, we proposed that the free A- and B-chain were derived from the enzymatic reduction of the interchain disulfide bond of cinnamomin. It was demonstrated that the endogenous type 11 RIPs of several plant species, such as Cinnamomum porrectum, Cinnamonium bodinieri and Ricinus communis, could be enzymatically reduced into the free A- and B-chain in their respective seed cells. The function of the free A-chain in the seed cell and the possibility that metabolic enzymes might be involved in the reduction of the interchain disulfide bond of type 11 RIPs in vivo are discussed. (C) 2003 Elsevier Science Ltd. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | RIBOSOME-INACTIVATING PROTEINS ; RICIN A-CHAIN ; EUKARYOTIC RIBOSOMES ; GLYCOSIDASE ACTIVITY ; DISULFIDE-ISOMERASE ; STORAGE PROTEIN ; SAMBUCUS-NIGRA ; RNA ; MECHANISM ; LECTIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000181383200008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2285]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hou, FJ,Xu, H,Liu, WY. Simultaneous existence of cinnamomin (a type II RIP) and small amount of its free A- and B-chain in mature seeds of camphor tree[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2003,35(4):455-464. |
| APA | Hou, FJ,Xu, H,&Liu, WY.(2003).Simultaneous existence of cinnamomin (a type II RIP) and small amount of its free A- and B-chain in mature seeds of camphor tree.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,35(4),455-464. |
| MLA | Hou, FJ,et al."Simultaneous existence of cinnamomin (a type II RIP) and small amount of its free A- and B-chain in mature seeds of camphor tree".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 35.4(2003):455-464. |
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