A peptide model of insulin folding intermediate with one disulfide
文献类型：期刊论文
| 作者 | Yan, H; Guo, ZY; Gong, XW; Xi, D; Feng, YM |
| 刊名 | PROTEIN SCIENCE
 |
| 出版日期 | 2003 |
| 卷号 | 12期号:4页码:768-775 |
| 关键词 | insulin folding disulfide kinetics thermodynamics |
| 通讯作者 | Feng, YM (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Insulin folds into a unique three-dimensional structure stabilized by three disulfide bonds. Our previous work suggested that during in vitro refolding of a recombinant single-chain insulin (PIP) there exists a critical folding intermediate containing the single disulfide A20-B19. However, the intermediate cannot be trapped during refolding because once this disulfide is formed, the remaining folding process is very quick. To circumvent this difficulty, a model peptide ([A20-B 19]PIP) containing the single disulfide A20-B 19 was prepared by protein engineering. The model peptide can be secreted from transformed yeast cells, but its secretion yield decreases 2-3 magnitudes compared with that of the wild-type PIP. The physicochemical property analysis suggested that the model peptide adopts a partially folded conformation. In vitro, the fully reduced model peptide can quickly and efficiently form the disulfide A20-B 19, which suggested that formation of the disulfide A20-B19 is kinetically preferred. In redox buffer, the model peptide is reduced gradually as the reduction potential is increased, while the disulfides of the wild-type PIP are reduced in a cooperative manner. By analysis of the model peptide, it is possible to deduce the properties of the critical folding intermediate with the single disulfide A20-B19. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | GROWTH-FACTOR-I ; MOLTEN GLOBULE ; ALPHA-LACTALBUMIN ; IGF-I ; MASS-SPECTROMETRY ; PROTEIN ; PATHWAY ; BONDS ; CYANYLATION ; PRECURSOR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000181646400014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2286]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yan, H,Guo, ZY,Gong, XW,et al. A peptide model of insulin folding intermediate with one disulfide[J]. PROTEIN SCIENCE,2003,12(4):768-775. |
| APA | Yan, H,Guo, ZY,Gong, XW,Xi, D,&Feng, YM.(2003).A peptide model of insulin folding intermediate with one disulfide.PROTEIN SCIENCE,12(4),768-775. |
| MLA | Yan, H,et al."A peptide model of insulin folding intermediate with one disulfide".PROTEIN SCIENCE 12.4(2003):768-775. |
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