Enhancement of germ cell apoptosis induced by ethanol in transgenic mice overexpressing Fas Ligand
文献类型：期刊论文
| 作者 | Hu, JH; Jiang, J; Ma, YH; Yang, N; Zhang, MH; Wu, M; Fei, J; Guo, LH |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2003 |
| 卷号 | 13期号:5页码:361-367 |
| 关键词 | Fas ligand ethanol apoptosis testes transgenic mouse |
| 通讯作者 | Zhang, MH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China., |
| 英文摘要 | It was suggested that chronic ethanol exposure could result in testicular germ cell apoptosis, but the mechanism is still unclear. In the present study, we use a model of transgenic mice ubiquitously overexpressing human FasL to investigate whether Fas ligand plays a role in ethanol-induced testicular germ cell apoptosis. Both wild-type (WT) mice and transgenic (TG) mice were treated with acute ethanol (20% v/v) by introperitoneal injection for five times. After ethanol injection, WT mice displayed up-regulation of Fas ligand in the testes, which was shown by FITC-conjugated flow cytometry and western blotting. Moreover, TG mice exhibited significantly more apoptotic germ cells than WT mice did after ethanol injection, which was demonstrated by DNA fragmentation, PI staining flow cytometry and TUNEL staining. In addition, histopathological examination revealed that degenerative changes of epithelial component of the tubules occurred in FasL overexpressing transgenic mice while testicular morphology was normal in wild-type mice after acute ethanol exposure, suggesting FasL expression determines the sensitivity of testes to ethanol in mice. In summary, we provide the direct evidences that Fas ligand mediates the apoptosis of testicular germ cells induced by acute ethanol using FasL transgenic mice. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | TESTICULAR INJURY ; IN-VITRO ; TESTES ; RAT ; SYSTEM ; DEATH ; SPERMATOGENESIS ; REGULATOR ; MECHANISM ; EXPOSURE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000187509700007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2292]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hu, JH,Jiang, J,Ma, YH,et al. Enhancement of germ cell apoptosis induced by ethanol in transgenic mice overexpressing Fas Ligand[J]. CELL RESEARCH,2003,13(5):361-367. |
| APA | Hu, JH.,Jiang, J.,Ma, YH.,Yang, N.,Zhang, MH.,...&Guo, LH.(2003).Enhancement of germ cell apoptosis induced by ethanol in transgenic mice overexpressing Fas Ligand.CELL RESEARCH,13(5),361-367. |
| MLA | Hu, JH,et al."Enhancement of germ cell apoptosis induced by ethanol in transgenic mice overexpressing Fas Ligand".CELL RESEARCH 13.5(2003):361-367. |
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