Purification and characterization of Moschatin, a novel type I ribosomeinactivating protein from the mature seeds of pumpkin (Cucurbita moschata), and preparation of its immunotoxin against human melanoma cells
文献类型：期刊论文
| 作者 | Xia, HC; Li, F; Li, Z; Zhang, ZC |
| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2003 |
| 卷号 | 13期号:5页码:369-374 |
| 关键词 | ribosome inactivating protein (RIP) Moschatin immunotoxin |
| 通讯作者 | Zhang, ZC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Proteom, Shanghai 200031, Peoples R China., |
| 英文摘要 | A novel ribosome-inactivating protein designated Moschatin from the mature seeds of pumpkin (Cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, CM-cellulose 52 column chromatography, Blue Sepharose CL-6B Affinity column chromatography and FPLC size-exclusion column chromatography. Moschatin is a type 1 RIP with a pI of 9.4 and molecular weight of similar to29 kD. It is a rRNA N-glycosidase and potently blocked the protein synthesis in the rabbit reticulocyte lysate with a IC50 of 0.26 nM. Using the anti-human melanoma McAb Ng76, a novel immunotoxin Moschatin-Ng76 was prepared successfully and it efficiently inhibited the growth of targeted melanoma cellsM(21) with a IC50 of 0.04 nM, 1500 times lower than that of free Moschatin. The results implied that Moschatin could be used as a new potential anticancer agent. |
| 学科主题 | Cell Biology |
| 类目[WOS] | Cell Biology |
| 关键词[WOS] | RIBOSOME-INACTIVATING PROTEINS ; EUKARYOTIC RIBOSOMES ; VITRO INHIBITION ; LUFFIN ; TRICHOSANTHIN ; CINNAMOMIN ; MECHANISM ; THERAPY ; TOXINS ; PLANTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000187509700008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2293]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xia, HC,Li, F,Li, Z,et al. Purification and characterization of Moschatin, a novel type I ribosomeinactivating protein from the mature seeds of pumpkin (Cucurbita moschata), and preparation of its immunotoxin against human melanoma cells[J]. CELL RESEARCH,2003,13(5):369-374. |
| APA | Xia, HC,Li, F,Li, Z,&Zhang, ZC.(2003).Purification and characterization of Moschatin, a novel type I ribosomeinactivating protein from the mature seeds of pumpkin (Cucurbita moschata), and preparation of its immunotoxin against human melanoma cells.CELL RESEARCH,13(5),369-374. |
| MLA | Xia, HC,et al."Purification and characterization of Moschatin, a novel type I ribosomeinactivating protein from the mature seeds of pumpkin (Cucurbita moschata), and preparation of its immunotoxin against human melanoma cells".CELL RESEARCH 13.5(2003):369-374. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。