Identification of GABA(B)R2 in rat testis and sperm
文献类型：期刊论文
| 作者 | He, XB; Zhang, YB; Yan, YC; Li, YP; Koide, SS |
| 刊名 | JOURNAL OF REPRODUCTION AND DEVELOPMENT
 |
| 出版日期 | 2003 |
| 卷号 | 49期号:5页码:397-402 |
| 关键词 | GABA(B)R2 testis sperm |
| 通讯作者 | Yan, YC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China.,ycyan@sunm.shcnc.ac.cn |
| 英文摘要 | GABA is capable of mimicking and potentiating the action of progesterone in initiating the acrosome reaction (AR) of mammalian sperm. The GABA-initiated AR is mediated by GABA(A)R; whereas GABA(B)R1 protein found in rat testis and sperm tends to modify this process. Moreover, the occurrence of GABA(B)R2, a subunit essential for the formation of a functionally active GABA(B)R, in rat testis and sperm has not been established. In the present study, rat testis and sperm were analyzed for the presence of GABA(B)R2 transcript and protein by RT-PCR, Northern blot, Western blot and an indirect immunofluorescence technique. Northern blot shows that the transcript of testis GABA(B)R2 is shorter (similar to3.0 Kb) than that of the brain (similar to5.6 Kb). The full length testis GABA(B)R2 cDNA was prepared by RACE-PCR and found to be shorter by 2.2 Kb in the segment at the extreme terminus of 3'UTR of rat brain GABA(B)R2 but, the sequences corresponding to the open reading frame and 5'-UTR of rat testis GABA(B)R2 were found to be identical to those of rat brain. GABA(B)R2 protein isolated from rat epididymal sperm was slighter larger than those of rat testis and brain. It was principally localized in the acrosome region of the head of rat sperm by an indirect immunofluorescence technique. The present results establish that GABA(B)R2 protein is produced in rat testis and sperm and may play a role in GABA triggering of AR. |
| 学科主题 | Agriculture; Reproductive Biology |
| 类目[WOS] | Agriculture, Dairy & Animal Science ; Reproductive Biology |
| 关键词[WOS] | GAMMA-AMINOBUTYRIC-ACID ; ACROSOME REACTION ; HUMAN SPERMATOZOA ; RECEPTOR ; PROGESTERONE ; GABA ; INVOLVEMENT ; EXOCYTOSIS ; EXPRESSION ; SUBUNITS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000185999700008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2294]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | He, XB,Zhang, YB,Yan, YC,et al. Identification of GABA(B)R2 in rat testis and sperm[J]. JOURNAL OF REPRODUCTION AND DEVELOPMENT,2003,49(5):397-402. |
| APA | He, XB,Zhang, YB,Yan, YC,Li, YP,&Koide, SS.(2003).Identification of GABA(B)R2 in rat testis and sperm.JOURNAL OF REPRODUCTION AND DEVELOPMENT,49(5),397-402. |
| MLA | He, XB,et al."Identification of GABA(B)R2 in rat testis and sperm".JOURNAL OF REPRODUCTION AND DEVELOPMENT 49.5(2003):397-402. |
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