Expression of human IFN alpha 2b-THY alpha 1 fused gene in Bombyx mori cell and study of its bioactivity
文献类型：期刊论文
| 作者 | Guo, DS; Zhu, CG; Zhang, YZ; Wu, XF |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2003 |
| 卷号 | 30期号:5页码:803-807 |
| 关键词 | IFN alpha 2b THY alpha 1 fused gene Bombyx mori nuclear polyhedrosis virus BmN cells gene expression |
| 通讯作者 | Zhang, YZ (reprint author), Zhejiang Univ, Inst Biochem, Hangzhou 310029, Peoples R China., |
| 英文摘要 | The baculovirus shuttle vector, pBacPAK-IFN-THY was constructed which contains the genes of IFN-alpha2b and THY-alpha1. Constructed vector was coinfected with linear Bm-BacPAK6 DNA into BmN cells. The recombinant virus was screened and plaque-purified. The BmN cells were infected with the recombinant virus. The results showed that the protein was successfully prepared and its bioactivities were testified by WISH-VSV system and E-RFC. Results indicated that the expressed fusion protein have bioactivities of both IFN-alpha2b and THY-alpha1. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ALPHA-1 |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000185930900022 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2296]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Guo, DS,Zhu, CG,Zhang, YZ,et al. Expression of human IFN alpha 2b-THY alpha 1 fused gene in Bombyx mori cell and study of its bioactivity[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2003,30(5):803-807. |
| APA | Guo, DS,Zhu, CG,Zhang, YZ,&Wu, XF.(2003).Expression of human IFN alpha 2b-THY alpha 1 fused gene in Bombyx mori cell and study of its bioactivity.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,30(5),803-807. |
| MLA | Guo, DS,et al."Expression of human IFN alpha 2b-THY alpha 1 fused gene in Bombyx mori cell and study of its bioactivity".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 30.5(2003):803-807. |
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