A novel function of Go alpha: mediation of extracellular signal-regulated kinase activation by opioid receptors in neural cells
文献类型：期刊论文
| 作者 | Zhang, WB; Zhang, Z; Ni, YX; Wu, YL; Pei, G |
| 刊名 | JOURNAL OF NEUROCHEMISTRY
 |
| 出版日期 | 2003 |
| 卷号 | 86期号:5页码:1213-1222 |
| 关键词 | delta opioid receptors dominant negative extracellular signal-regulated kinase Go alpha RNA interference |
| 通讯作者 | Pei, G (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Go is the most abundant G protein expressed in brain but its function is less known. Here we show a novel function of Goalpha as a mediator of opioid receptor-induced extracellular signal-regulated kinase activation in neural cells. The current study found that, in neuroblastoma x glioma NG108-15 hybrid cells, activation of extracellular signal-regulated kinase through delta opioid receptors was mediated by pertussis toxin-sensitive G protein and independent of Gbetagamma subunits, PI3 kinase and receptor internalization. Overexpression of a dominant negative form of Goalpha(1) , but not Gialpha(2) , completely blocked delta opioid receptor-induced extracellular signal-regulated kinase activity. Decreasing Goalpha expression by RNA interference greatly reduced delta opioid receptor-induced extracellular signal-regulated kinase activity and extracellular signal-regulated kinase-dependent gene expression, while knocking down Gialpha(2) did not. By taking advantage of differences between human and mouse Goalpha gene sequences, we simultaneously knocked down endogenous Goalpha expression and expressed exogenous human Goalpha subunits. We found that both human Goalpha(1) and Goalpha(2) could mediate delta opioid receptor-induced extracellular signal-regulated kinase activation. This study suggests that one of the functions of Goalpha in the brain is to mediate extracellular signal-regulated kinase activation by G protein-coupled receptors. |
| 学科主题 | Biochemistry & Molecular Biology; Neurosciences & Neurology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Neurosciences |
| 关键词[WOS] | GTP-BINDING PROTEIN ; G-BETA-GAMMA ; DEPENDENT MECHANISM ; NUCLEOTIDE-BINDING ; MAPK ACTIVATION ; P38 MAPK ; 2 FORMS ; SUBUNIT ; PATHWAY ; INTERNALIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000184723000016 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2299]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, WB,Zhang, Z,Ni, YX,et al. A novel function of Go alpha: mediation of extracellular signal-regulated kinase activation by opioid receptors in neural cells[J]. JOURNAL OF NEUROCHEMISTRY,2003,86(5):1213-1222. |
| APA | Zhang, WB,Zhang, Z,Ni, YX,Wu, YL,&Pei, G.(2003).A novel function of Go alpha: mediation of extracellular signal-regulated kinase activation by opioid receptors in neural cells.JOURNAL OF NEUROCHEMISTRY,86(5),1213-1222. |
| MLA | Zhang, WB,et al."A novel function of Go alpha: mediation of extracellular signal-regulated kinase activation by opioid receptors in neural cells".JOURNAL OF NEUROCHEMISTRY 86.5(2003):1213-1222. |
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