Secretory expression of an antifungal protein in the mould Trichoderma viride
文献类型：期刊论文
| 作者 | Xu, J; Gong, ZZ |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:5页码:454-458 |
| 关键词 | Aspergillus giganteus Trichoderma viride secret expression |
| 通讯作者 | , |
| 英文摘要 | AFP (antifugal protein) secreted by the filamentous fungi Aspergillus giganteus MDH18894 is a strong basic protein (pl > 10.65). Its mRNA has an 430 bp open reading frame encoding the 94 amino acids' precursor of AFP. The precursor of AFP is processed to form the mature AFP of 51 amino acids by a putative two-step processing. A 285 bp AFP-like gene (i.e. AFP cDNA) lacking introns was reported to exist in the genome of Trichoderma viride, another species of filamentous fungi, but the gene was silent. In order to investigate whether the genomic AFP gene from A. giganteus could be expressed in T. viride, an expression plasmid With the open reading frame of AFP gene fused between the promoter and terminator of trpC gene was constructed and was transformed into T. viride. SDS-PAGE and Western-blot analysis showed that AFP was expressed and secreted into the culture supernatant of the transformant of T. viride. Our study provides a basis for further secretory expression of heterologous eukaryotic genes of important value in the system of T. viride. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ASPERGILLUS-GIGANTEUS ; GENE-EXPRESSION ; INTRON REQUIREMENT ; TRANSFORMATION ; PROMOTER ; NIDULANS ; AFP |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000183076800010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2302]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xu, J,Gong, ZZ. Secretory expression of an antifungal protein in the mould Trichoderma viride[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(5):454-458. |
| APA | Xu, J,&Gong, ZZ.(2003).Secretory expression of an antifungal protein in the mould Trichoderma viride.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(5),454-458. |
| MLA | Xu, J,et al."Secretory expression of an antifungal protein in the mould Trichoderma viride".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.5(2003):454-458. |
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