Contribution of the absolutely conserved B8Gly to the foldability of insulin
文献类型：期刊论文
| 作者 | Guo, ZY; Tang, YH; Wang, S; Feng, YM |
| 刊名 | BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2003 |
| 卷号 | 384期号:5页码:805-809 |
| 关键词 | disulfide foldability insulin refolding stability |
| 通讯作者 | Feng, YM (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China., |
| 英文摘要 | B8Gly is absolutely conserved in insulin from different species, and in other members of the insulin superfamily the corresponding position is always occupied by a Gly residue. However, the reasons for its conservation are still unclear; probably many factors contribute to this phenomenon. In our previous work, B8Gly was replaced by an Ala residue, which suggested that biological activity is one of the factors contributing to its conservation. In order to identify more factors contributing to this positional conservation, the secretion efficiency, structural stability, disulfide stability, and in vitro refolding of single-chain insulin (PIP) and a mutant with B8Gly replaced by Ala, were investigated. Compared with wild-type PIP, the B8Ala replacement decreased the secretion efficiency, structural stability, disulfide stability, and in vitro refolding efficiency of the PIP sequence. These results suggest that B8Gly is important to the secretion, folding, and stability of the insulin sequence. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | DISULFIDE BRIDGE ; PROTEIN ; PRECURSOR ; MOLECULE ; PATHWAY ; MONOMER ; CHAINS ; ANALOG ; DESIGN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000183421000013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2303]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Guo, ZY,Tang, YH,Wang, S,et al. Contribution of the absolutely conserved B8Gly to the foldability of insulin[J]. BIOLOGICAL CHEMISTRY,2003,384(5):805-809. |
| APA | Guo, ZY,Tang, YH,Wang, S,&Feng, YM.(2003).Contribution of the absolutely conserved B8Gly to the foldability of insulin.BIOLOGICAL CHEMISTRY,384(5),805-809. |
| MLA | Guo, ZY,et al."Contribution of the absolutely conserved B8Gly to the foldability of insulin".BIOLOGICAL CHEMISTRY 384.5(2003):805-809. |
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