Effect of specific nucleotide G(11) on cleavage activity of hairpin ribozymes in vitro
文献类型：期刊论文
| 作者 | Song, YH; Lin, JS; Kong, XJ; Li, B; Liu, NZ; Jin, YX |
| 刊名 | PROGRESS IN NATURAL SCIENCE
 |
| 出版日期 | 2003 |
| 卷号 | 13期号:5页码:345-349 |
| 关键词 | hairpin ribozyme kinetic of enzyme point mutant |
| 通讯作者 | Jin, YX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | To study the effect of specific G(11) nucleotide on cleavage activity of hairpin ribozyme in vitro, the P-32-labeled pKC transcript (267 nt) containing hepatitis B virus core region is used as target RNA. Synthetic hairpin ribozyme genes with G(11) mutational site are cloned into ribozyme vector p1.5 to create pHpRz for preparation of ribozyme. Non-labeled HpRz transcripts are incubated with P-32- labeled target-RNAs under different conditions and autoradiographed after gel electrophoresis. The results show that the cleavage activity of hairpin ribozyme is closely related to the matching of the bases between hairpin ribozyme and the substrate. The G(11) site is not essential to cleavage activity of hairpin ribozyme in vitro, so the selection of target sequence matching G(11) site of hairpin ribozyme is not limited to this site. |
| 学科主题 | Materials Science; Science & Technology - Other Topics |
| 类目[WOS] | Materials Science, Multidisciplinary ; Multidisciplinary Sciences |
| 关键词[WOS] | TOBACCO RINGSPOT VIRUS ; IN-VITRO ; RNA ; INHIBITION ; SELECTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000182724600005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2307]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Song, YH,Lin, JS,Kong, XJ,et al. Effect of specific nucleotide G(11) on cleavage activity of hairpin ribozymes in vitro[J]. PROGRESS IN NATURAL SCIENCE,2003,13(5):345-349. |
| APA | Song, YH,Lin, JS,Kong, XJ,Li, B,Liu, NZ,&Jin, YX.(2003).Effect of specific nucleotide G(11) on cleavage activity of hairpin ribozymes in vitro.PROGRESS IN NATURAL SCIENCE,13(5),345-349. |
| MLA | Song, YH,et al."Effect of specific nucleotide G(11) on cleavage activity of hairpin ribozymes in vitro".PROGRESS IN NATURAL SCIENCE 13.5(2003):345-349. |
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