cDNA cloning of two A-superfamily conotoxins from Conus striatus
文献类型：期刊论文
| 作者 | Wang, CZ; Jiang, H; Ou, ZL; Chen, JS; Chi, CW |
| 刊名 | TOXICON
 |
| 出版日期 | 2003 |
| 卷号 | 42期号:6页码:613-619 |
| 关键词 | conotoxin Conus striatus Conus peptide cDNA cloning |
| 通讯作者 | Chi, CW (reprint author), Chinese Acad Sci, Key Lab Proteom, Shanghai Inst Biol Sci, Shanghai Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | The full-length cDNAs of two A-superfamily conotoxins, kappaA-SIVA and alpha-SII, were respectively cloned and sequenced from Conus striatus using 3' RACE and 5' RACE. The cDNA of kappaA-SIVA encodes a precursor of 68 residues, including a signal peptide of 21 residues, a pro-peptide of 17 residues, and a mature peptide of 30 residues with an additional residue Gly which is prerequisite for the amidation of the preceding C-terminal Cys. The cDNA-deduced sequence of alpha-SII is composed of a signal peptide of 21 residues, a pro-peptide of 29 residues, a mature peptide of 19 residues and three additional residues Arg-Thr-Ile at the C-terminus. This tripeptide might be cleaved off by proteolytic processing. Although these two conotoxins belong to different families and target voltage-gated potassium channel and nicotinic acetylcholine receptor, respectively, they share the same signal sequence, and both are processed at the common signal site -X-Arg- immediately before the mature peptide sequences. The length of 3' untranslational region of a-conotoxin SII was extraordinarily large about 10 times longer than that of kappaA-SIVA with 770 and 75 bp, respectively. The elucidated cDNAs of these two toxins will facilitate a better understanding of the process of their post-translational modifications. (C) 2003 Elsevier Ltd. All rights reserved. |
| 学科主题 | Pharmacology & Pharmacy; Toxicology |
| 类目[WOS] | Pharmacology & Pharmacy ; Toxicology |
| 关键词[WOS] | BUTHUS-MARTENSI KARSCH ; NICOTINIC ACETYLCHOLINE-RECEPTORS ; GENOMIC ORGANIZATION ; POTASSIUM CHANNELS ; OMEGA-CONOTOXIN ; ALPHA-CONOTOXIN ; SODIUM-CHANNELS ; ION-CHANNEL ; PEPTIDE ; SNAILS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000186673300005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2313]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, CZ,Jiang, H,Ou, ZL,et al. cDNA cloning of two A-superfamily conotoxins from Conus striatus[J]. TOXICON,2003,42(6):613-619. |
| APA | Wang, CZ,Jiang, H,Ou, ZL,Chen, JS,&Chi, CW.(2003).cDNA cloning of two A-superfamily conotoxins from Conus striatus.TOXICON,42(6),613-619. |
| MLA | Wang, CZ,et al."cDNA cloning of two A-superfamily conotoxins from Conus striatus".TOXICON 42.6(2003):613-619. |
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