The phosphorylation of NS protein of wheat rosette stunt virus
文献类型：期刊论文
| 作者 | Xie, BT; Ye, YJ; Gong, ZX |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:6页码:518-521 |
| 关键词 | wheat rosette stunt virus NS protein phosphorylation protein kinase |
| 通讯作者 | , |
| 英文摘要 | The genome of wheat rosette stunt virus (WRSV), a plant rhabdovirus, is a single negative strand RNA. It encodes five viral structural proteins: the glycoprotein (G), the matrix protein (M), the nucleocapsid protein (N), the large protein (L) and the non-structural protein (NS), which was later proved to be a viral structural protein too and existed in a variety of phosphorylation forms in case of vascular stomatitis virus (VSV). In this paper we demonstrated that NS protein of WRSV, either bound with the viral nucleocapsid or expressed in bacteria could be in vitro phosphorylated in presence of viral nucleocapsid. We concluded that the NS protein of WRSV was a phosphorylated protein and it might exist in both phosphorylated and dephosphorylated forms in virions. Our results excluded the possibility that the NS protein could be autophosphorylated. The L protein, the major component of viral RNA dependent RNA polymerase is associated with the protein kinase for phosphorylation of NS protein. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | VESICULAR STOMATITIS-VIRUS ; RNA-POLYMERASE ; PHOSPHOPROTEIN ; GENE ; TRANSCRIPTION ; REPLICATION ; EXPRESSION ; CLONING ; DOMAIN ; INVITRO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000183733400005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2314]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xie, BT,Ye, YJ,Gong, ZX. The phosphorylation of NS protein of wheat rosette stunt virus[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(6):518-521. |
| APA | Xie, BT,Ye, YJ,&Gong, ZX.(2003).The phosphorylation of NS protein of wheat rosette stunt virus.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(6),518-521. |
| MLA | Xie, BT,et al."The phosphorylation of NS protein of wheat rosette stunt virus".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.6(2003):518-521. |
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