Purification and characterization of Luffin P1, a ribosome-inactivating peptide from the seeds of Luffa cylindrica
文献类型：期刊论文
| 作者 | Li, F; Yang, XX; Xia, HC; Zeng, R; Hu, WG; Li, Z; Zhang, ZC |
| 刊名 | PEPTIDES
 |
| 出版日期 | 2003 |
| 卷号 | 24期号:6页码:799-805 |
| 关键词 | Luffin P1 ribosome-inactivating protein N-glycosidase Luffa cylindrica polymerization |
| 通讯作者 | Zhang, ZC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Proteom, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | A peptide designated Luffin P1 was purified from the seeds of Luffa cylindrica. Its molecular mass was determined to be 5226.1 Da by MALDI-TOF MS analysis. The purified Luffin P1 shows a strong inhibitory activity on protein synthesis in the cell-free rabbit reticulocyte lysate with IC50 of 0.88 nM. Its reaction mechanism is the same as that of the ribosome-inactivating protein trichosanthin, which is an rRNA N-glycosidase. Besides, the results of gel filtration chromatography suggested the existence of polymers of Luffin P1 and polymerization of Luffin P1 enhanced its rRNA N-glycosidase activity. Luffin P1 was the smallest peptide yet reported that has translational inhibitory activity. The cDNA and deduced amino acid sequence of Luffin P1 has also been determined. (C) 2003 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Pharmacology & Pharmacy |
| 类目[WOS] | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| 关键词[WOS] | GOURD MOMORDICA-CHARANTIA ; N-GLYCOSIDASE ACTIVITY ; RICIN A-CHAIN ; PROTEIN ; MECHANISM ; IMMUNOTOXIN ; RNA ; MOMORCHARIN ; INHIBITION ; INTEGRASE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000185266800002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2320]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, F,Yang, XX,Xia, HC,et al. Purification and characterization of Luffin P1, a ribosome-inactivating peptide from the seeds of Luffa cylindrica[J]. PEPTIDES,2003,24(6):799-805. |
| APA | Li, F.,Yang, XX.,Xia, HC.,Zeng, R.,Hu, WG.,...&Zhang, ZC.(2003).Purification and characterization of Luffin P1, a ribosome-inactivating peptide from the seeds of Luffa cylindrica.PEPTIDES,24(6),799-805. |
| MLA | Li, F,et al."Purification and characterization of Luffin P1, a ribosome-inactivating peptide from the seeds of Luffa cylindrica".PEPTIDES 24.6(2003):799-805. |
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