Ref-1 protein enhances the IL-2-stimulated telomerase activity
文献类型：期刊论文
| 作者 | Xu, WJ; Yan, MD; Sun, LY; Zheng, ZC; Liu, XY |
| 刊名 | JOURNAL OF CELLULAR BIOCHEMISTRY
 |
| 出版日期 | 2003 |
| 卷号 | 88期号:6页码:1120-1128 |
| 关键词 | IL-2 Ref-1 telomerase redox regulation |
| 通讯作者 | Liu, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 YueYang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Telomerase is an important ribonucleoprotein enzyme involved in cellular proliferation and senescence. Activation of telomerase has been detected in a vast majority of human cancer cells. In this article, we demonstrated that Interleukin-2 (IL-2) which is the pivotal cytokine in the immune system could stimulate the activity of telomerase in the cultured BA/F3beta cells. It was also found that the level of IL-2-induced telomerase activity was decreased by the treatment with chemical oxidant in vitro. Since IL-2 stimulation produces a oxidative shift of the intracellular environment, the activation and maintenance of telomerase in this oxidative circumstance requires particular protection. Here we proved the redox factor-1 (Ref-1) protein was involved in this process. The addition of GST-Ref-1 protein increased the level of IL-2-induced telomerase activity in the TRAP assay, while elimination of the endogenous Ref-1 protein by immunodepletion decreased it. Consistent with these in vitro results, IL-2-induced telomerase activity could be enhanced by transient overexpression of Ref-1 protein in BA/F3beta cells. Taken together, these findings proved that Ref-1 protein benefits the activation of telomerase activity in the oxidative microenvironment of the BA/F3beta cells stimulated by IL-2. (C) 2003 Wiley-Liss, Inc. |
| 学科主题 | Biochemistry & Molecular Biology; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Cell Biology |
| 关键词[WOS] | VITAMIN-C ; T-CELLS ; ACTIVATION ; HYPOXIA ; CANCER ; INTERLEUKIN-2 ; IL-2 ; GENE ; AP-1 ; P53 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000181999100006 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2322]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Xu, WJ,Yan, MD,Sun, LY,et al. Ref-1 protein enhances the IL-2-stimulated telomerase activity[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2003,88(6):1120-1128. |
| APA | Xu, WJ,Yan, MD,Sun, LY,Zheng, ZC,&Liu, XY.(2003).Ref-1 protein enhances the IL-2-stimulated telomerase activity.JOURNAL OF CELLULAR BIOCHEMISTRY,88(6),1120-1128. |
| MLA | Xu, WJ,et al."Ref-1 protein enhances the IL-2-stimulated telomerase activity".JOURNAL OF CELLULAR BIOCHEMISTRY 88.6(2003):1120-1128. |
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