中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Multi-strips on one gel method to improve the reproducibility, resolution power and high-throughput of two-dimensional electrophoresis

文献类型:期刊论文

作者Yuan, Q; An, J; Liu, DG; Zhao, FK
刊名ACTA BIOCHIMICA ET BIOPHYSICA SINICA
出版日期2003
卷号35期号:7页码:611-618
关键词two-dimensional electrophoresis multi-strips one gel (MSOG) method reproducibility high-throughput proteomics
通讯作者Zhao, FK (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Proteom, Shanghai 200031, Peoples R China.,
英文摘要Two-dimensional polyacrylamide gel electrophoresis is one of the most key separation tools which can reveal hundreds or even thousands of proteins at a time in proteomic research. In this paper, we report a new IPG strip application, called mufti-strips on one gel (MSOG) method. By comparing the 2-DE patterns of the same sample, the different state samples and the same sample in the different second dimensional SDS running systems (large size and medium size gels), we found this new method can not only improve the reproducibility and resolution power of 2-DE pattern, but also achieve. high throughput and economical format which is helpful to automatic proteomic research.
学科主题Biochemistry & Molecular Biology; Biophysics
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]ASSISTED-LASER-DESORPTION/IONIZATION ; IMMOBILIZED PH GRADIENTS ; PROTEIN-DETECTION ; SYPRO RUBY ; IDENTIFICATION ; PROTEOMICS ; PROTOCOL ; STAINS
收录类别SCI
语种英语
WOS记录号WOS:000184466500004
版本出版稿
源URL[http://202.127.25.143/handle/331003/2323]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714
Yuan, Q,An, J,Liu, DG,et al. Multi-strips on one gel method to improve the reproducibility, resolution power and high-throughput of two-dimensional electrophoresis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(7):611-618.
APA Yuan, Q,An, J,Liu, DG,&Zhao, FK.(2003).Multi-strips on one gel method to improve the reproducibility, resolution power and high-throughput of two-dimensional electrophoresis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(7),611-618.
MLA Yuan, Q,et al."Multi-strips on one gel method to improve the reproducibility, resolution power and high-throughput of two-dimensional electrophoresis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.7(2003):611-618.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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