Multi-strips on one gel method to improve the reproducibility, resolution power and high-throughput of two-dimensional electrophoresis
文献类型：期刊论文
| 作者 | Yuan, Q; An, J; Liu, DG; Zhao, FK |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:7页码:611-618 |
| 关键词 | two-dimensional electrophoresis multi-strips one gel (MSOG) method reproducibility high-throughput proteomics |
| 通讯作者 | Zhao, FK (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Proteom, Shanghai 200031, Peoples R China., |
| 英文摘要 | Two-dimensional polyacrylamide gel electrophoresis is one of the most key separation tools which can reveal hundreds or even thousands of proteins at a time in proteomic research. In this paper, we report a new IPG strip application, called mufti-strips on one gel (MSOG) method. By comparing the 2-DE patterns of the same sample, the different state samples and the same sample in the different second dimensional SDS running systems (large size and medium size gels), we found this new method can not only improve the reproducibility and resolution power of 2-DE pattern, but also achieve. high throughput and economical format which is helpful to automatic proteomic research. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ASSISTED-LASER-DESORPTION/IONIZATION ; IMMOBILIZED PH GRADIENTS ; PROTEIN-DETECTION ; SYPRO RUBY ; IDENTIFICATION ; PROTEOMICS ; PROTOCOL ; STAINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000184466500004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2323]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yuan, Q,An, J,Liu, DG,et al. Multi-strips on one gel method to improve the reproducibility, resolution power and high-throughput of two-dimensional electrophoresis[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(7):611-618. |
| APA | Yuan, Q,An, J,Liu, DG,&Zhao, FK.(2003).Multi-strips on one gel method to improve the reproducibility, resolution power and high-throughput of two-dimensional electrophoresis.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(7),611-618. |
| MLA | Yuan, Q,et al."Multi-strips on one gel method to improve the reproducibility, resolution power and high-throughput of two-dimensional electrophoresis".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.7(2003):611-618. |
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