Thermodynamic and kinetic analysis of unfolding of P23k protein isolated from spinach photosystem II
文献类型：期刊论文
| 作者 | Tan, CY; Xu, CH; Shen, JR; Sukuma, S; Yamamoto, Y; Balny, C; Ruan, KC |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:7页码:677-681 |
| 关键词 | fluorescence spectroscopy high pressure technique protein unfolding |
| 通讯作者 | Ruan, KC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Proteom, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | The unfolding of 23kD (P23k) protein isolated from spinach photosystem II particle was studied by high pressure and fluorescence spectroscopy. The thermal equilibrium study indicated that the protein could be totally unfolded by 180 or 160 MPa at 20 degreesC and 3 degreesC, respectively. The standard free energy and standard volume change of the protein for unfolding at 20 degreesC is 23.45 kJ/mol and - 150.3 ml/mol, respectively. Kinetics study indicated that at 20 degreesC the activation volume for unfolding, Delta V-u(double dagger), was negative ( - 66.2 ml/mol), meanwhile the activation volume for folding, Delta V-f(double dagger), was positive (84.1 ml/mol). The rate constants for folding and unfolding (K-0f, K-0u) were 1.87 s(-1) and 1.3 x 10(-4) s(-1), respectively, these results provide some clues to explain why the protein is so sensitive to pressure. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | OXYGEN-EVOLVING COMPLEX ; HIGH-PRESSURE ; DENATURATION ; TRYPSIN |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000184466500015 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2324]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Tan, CY,Xu, CH,Shen, JR,et al. Thermodynamic and kinetic analysis of unfolding of P23k protein isolated from spinach photosystem II[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(7):677-681. |
| APA | Tan, CY.,Xu, CH.,Shen, JR.,Sukuma, S.,Yamamoto, Y.,...&Ruan, KC.(2003).Thermodynamic and kinetic analysis of unfolding of P23k protein isolated from spinach photosystem II.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(7),677-681. |
| MLA | Tan, CY,et al."Thermodynamic and kinetic analysis of unfolding of P23k protein isolated from spinach photosystem II".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.7(2003):677-681. |
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