Identification of the amino acid residues in trichosanthin crucial for IgE response
文献类型：期刊论文
| 作者 | Cai, X; Yao, G; Xu, GF; Yang, CH; Xu, H; Lin, Y; Yu, J; Sun, B |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2002 |
| 卷号 | 297期号:3页码:510-516 |
| 关键词 | trichosanthin allergen IgE-epitope phage-peptide library mutation |
| 通讯作者 | Sun, B (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Mol Immunol, 320 Yueyang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Trichosanthin (TCS) is the major effective component from Chinese herb Trichosanthes kirilowii. TCS has been approved to be effective in clinical treatment of HIV infection and leukemia, but its allergenicity has limited its clinical usage. To identify amino acid residues in TCS with an important role in IgE induction, TCS-specific IgE mAb (TE1) was used to serve as a probe and TE1 epitope was determined by a random phage-peptide library. Based on phage peptide sequences, TE1 epitope was predicted at amino acid residues 169-174 (QQIGKR) of TCS protein. Based on modeling data, two amino acids (Lys173 and Arg174) on TCS were considered to have a crucial role in binding to TE1 After lysine 173 and arginine 174 were mutated to glycine, the mutant TCS protein specifically lost the binding activity to TE1 mAb and exhibited reduced IgE induction in the immunized mice. The data showed that the IgE epitope of TCS was determined and shown to play a critical role in induction of IgE, and the modification of IgE-epitope may be a useful strategy to reduce the allergenicity of an allergen. (C) 2002 Elsevier Science (USA). All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | PEPTIDE LIBRARY ; ALLERGEN ; COMPLEX ; EPITOPE ; GLQ223 ; HIV ; REPLICATION ; MACROPHAGES ; RESOLUTION ; ACTIVATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000178380500016 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2327]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Cai, X,Yao, G,Xu, GF,et al. Identification of the amino acid residues in trichosanthin crucial for IgE response[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2002,297(3):510-516. |
| APA | Cai, X.,Yao, G.,Xu, GF.,Yang, CH.,Xu, H.,...&Sun, B.(2002).Identification of the amino acid residues in trichosanthin crucial for IgE response.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,297(3),510-516. |
| MLA | Cai, X,et al."Identification of the amino acid residues in trichosanthin crucial for IgE response".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 297.3(2002):510-516. |
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