Production and application of rabbit anti-imitative spider dragline silk protein polyclonal antibody
文献类型：期刊论文
| 作者 | Guo, TQ; Zhao, Y; Wang, SP; Dong, L; Lu, CD |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:8页码:756-760 |
| 关键词 | synthetic spider dragline rabbit anti-serum cocoon ELISA |
| 通讯作者 | Lu, CD (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | A synthetic spider dragline gene s600 was cloned into fusion protein expression vector pGEX-KG and expressed in Escherichia coli. Protein S600 was purified and rabbit antiserum was prepared. Amino acid composition analysis confirmed the right expression of S600. Western blot analysis revealed that anti-S600 antiserum. could react with natural spider silk, so the synthetic dragline protein, designed by the authors, shares similar immunological characteristics with the natural spider silk. An ELISA system was also established for the quantitative detection of synthetic dragline protein expression in silk gland (or in the cocoon) of transgenic silkworm. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ESCHERICHIA-COLI ; FIBROIN ; FIBER |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000184988600013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2331]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Guo, TQ,Zhao, Y,Wang, SP,et al. Production and application of rabbit anti-imitative spider dragline silk protein polyclonal antibody[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(8):756-760. |
| APA | Guo, TQ,Zhao, Y,Wang, SP,Dong, L,&Lu, CD.(2003).Production and application of rabbit anti-imitative spider dragline silk protein polyclonal antibody.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(8),756-760. |
| MLA | Guo, TQ,et al."Production and application of rabbit anti-imitative spider dragline silk protein polyclonal antibody".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.8(2003):756-760. |
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