Expression and characterization of Kingle 1-5 domains of human plasminogen
文献类型：期刊论文
| 作者 | Zhou, QW; Xie, JL; Xin, L; Ye, Q; Li, ZP; Gan, RB |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:8页码:761-767 |
| 关键词 | K1-5 Pichia pastoris expression purification bioactivity |
| 通讯作者 | Gan, RB (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | The cDNA encoding Kringle 1 - 5 domains of human plasminogen (designated as K1 - 5), obtained from HepG2 by RT-PCR, was cloned into expression vector pHIL-S1. The recombinant plasmid pHIL-K1 - 5 was transformed into Pichia pastoris GS115 and the recombinant yeast was induced by methanol to express the recombinant protein. The expressed protein was purified by lysine affinity chromatography. The recombinant K1 - 5 inhibited the growth of bovine aortic endothelial cells (BAEC) stimulated by the basic fibroblast growth factor (bFGF), in a dosage-dependent manner, with a half maximal concentration of 14 mg/L. And rhK1 - 5 inhibited 47% of the BAEC migration stimulated by bFGF at the concentration of 50 mg/L. rhK1 - 5 also affected the cell cycle of BAEC and caused G(0)-G(1) arrest at the concentration of 14 mg/L. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | SUPPRESSES TUMOR-GROWTH ; PICHIA-PASTORIS ; ANTIANGIOGENIC ACTIVITY ; ANGIOGENESIS INHIBITOR ; RECOMBINANT PROTEINS ; BASEMENT-MEMBRANE ; HUMAN ANGIOSTATIN ; COLLAGEN ; ENDOSTATIN ; KRINGLE-5 |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000184988600014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2332]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhou, QW,Xie, JL,Xin, L,et al. Expression and characterization of Kingle 1-5 domains of human plasminogen[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(8):761-767. |
| APA | Zhou, QW,Xie, JL,Xin, L,Ye, Q,Li, ZP,&Gan, RB.(2003).Expression and characterization of Kingle 1-5 domains of human plasminogen.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(8),761-767. |
| MLA | Zhou, QW,et al."Expression and characterization of Kingle 1-5 domains of human plasminogen".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.8(2003):761-767. |
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