Expression and purification of recombinant SARS coronavirus spike protein
文献类型：期刊论文
| 作者 | Yu, H; Yang, Y; Zhang, W; Xie, YH; Qin, J; Wang, Y; Zheng, HB; Zhao, GP; Yang, S; Jiang, WH |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:8页码:774-778 |
| 关键词 | severe acute respiratory syndrome (SARS) coronavirus spike protein expression |
| 通讯作者 | Yang, S (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China., |
| 英文摘要 | A novel coronavirus (SARS-coronavirus, SARS-CoV) was discovered in association with cases of severe acute respiratory syndrome (SARS) recently. The first step in coronavirus infection is binding of the viral spike protein to certain receptor on host cells. The spike protein is the main surface antigen of the coronavirus and there should be antibodies against spike protein in patients' serum. Thus, to develop and expression protein fragment from spike protein gene are the purposes of this experiment. Partial spike gene fragments (751 similar to 1925 bp, 2005 similar to 34 10 bp, 1 similar to 1925 bp and 32 similar to 3659 bp) and its intact gene were cloned into pET32 or pGEX vectors, and transformed into competent Escherichia coli BL21 (DE3) (pLysS), respectively. 63, 78, 98, 160 and 164 kD fusion proteins were successfully expressed with amounts of 35%, 34%, 24%, 17% and 5% of total cell protein. The soluble parts of the cell crude extract were then partially purified by GST affinity chromatography. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | ACUTE RESPIRATORY SYNDROME ; ANTIBODIES ; SEQUENCE |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000184988600016 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2334]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yu, H,Yang, Y,Zhang, W,et al. Expression and purification of recombinant SARS coronavirus spike protein[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(8):774-778. |
| APA | Yu, H.,Yang, Y.,Zhang, W.,Xie, YH.,Qin, J.,...&Jiang, WH.(2003).Expression and purification of recombinant SARS coronavirus spike protein.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(8),774-778. |
| MLA | Yu, H,et al."Expression and purification of recombinant SARS coronavirus spike protein".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.8(2003):774-778. |
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