Inhibition of replication of goose paramyxovirus SF02 by hammerhead ribozyme targeting to the SF02F mRNA in chicken embryo fibroblasts
文献类型：期刊论文
| 作者 | Zou, J; Gong, ZX |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:9页码:801-805 |
| 关键词 | hammerhead ribozyme goose paramyxovirus NDV chicken embryo fibroblasts |
| 通讯作者 | Gong, ZX (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Proteom, Shanghai 200031, Peoples R China., |
| 英文摘要 | Hammerhead ribozyme RzF598 and its dysfunctional mutant dRzF598 targeting to the F gene of goose paramyxovirus SF02 have been designed. The transgenic plasmids pcDNA-RzF598 and pcDNA-dRzF598 were constructed by inserting ribozyme genes into eukaryotic expression vector pcDNA3. The plasmid pcDNA3 that lacks full ribozyme gene was used as a control. Plasmids pcDNA-RzF598, pcDNA-dRzF598 and pcDNA3 were transfected into chicken embryo fibroblasts (CEFs). The concentration of virus released by infected CEFs and the survival percentages of CEFs were identified. The results indicated that RzF598 successfully suppressed the replication of SF02 in CEFs. Survival percentage of CEFs being transfected with pcDNA-RzF598 and infected SF02 was up to 78.8%, while the survival percentages of untransfected CEFs and CEFs transfected with pcDNA3 after infection with SF02 were only about 5%. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | NEWCASTLE-DISEASE-VIRUS ; MULTIPLEX RT-PCR ; PHYLOGENETIC-RELATIONSHIPS ; NUCLEOTIDE-SEQUENCE ; FUSION PROTEIN ; CLEAVAGE ; ISOLATE ; GENE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000185493600004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2341]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zou, J,Gong, ZX. Inhibition of replication of goose paramyxovirus SF02 by hammerhead ribozyme targeting to the SF02F mRNA in chicken embryo fibroblasts[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(9):801-805. |
| APA | Zou, J,&Gong, ZX.(2003).Inhibition of replication of goose paramyxovirus SF02 by hammerhead ribozyme targeting to the SF02F mRNA in chicken embryo fibroblasts.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(9),801-805. |
| MLA | Zou, J,et al."Inhibition of replication of goose paramyxovirus SF02 by hammerhead ribozyme targeting to the SF02F mRNA in chicken embryo fibroblasts".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.9(2003):801-805. |
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