Purification and characterization of trichokirin-S1, a novel ribosome-inactivating peptide from seeds of Trichosanthes kirilowii
文献类型：期刊论文
| 作者 | Li, F; Yang, XX; Hu, WG; Xia, HC; Li, Z; Zhang, ZC |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:9页码:841-846 |
| 关键词 | trichokirin-S1 translational inhibitory activity rRNA N-glycosidase |
| 通讯作者 | Zhang, ZC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Proteom, Shanghai 200031, Peoples R China., |
| 英文摘要 | A novel peptide from the seeds of Trichosanthes kirilowii, trichokirin-S1, was purified by extraction of protein body, ammonia sulfate precipitation, Blue-gel affinity chromatography, FPLC Mono S ion exchange chromatography and Superose12 gel filtration chromatography. Its molecular weight was determined to be 11 426 by MALDI-TOF MS analysis. Its reaction mechanism to inactive ribosome was the same as that of the ribosome-inactivating protein trichosanthin, a rRNA N-glycosidase. The purified trichokirin-SI showed a strong inhibitory activity on protein synthesis in cell-free rabbit reticulocyte lysate system, with IC50 of 0.7 nmol/L. Therefore, trichokirin-SI may be a promising and efficient toxin moiety of immunotoxins. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | TRANSLATION-INHIBITING ACTIVITY ; VACUOLAR PROCESSING ENZYME ; BETA-KIRILOWIN ; PROTEIN ; MECHANISM |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000185493600011 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2343]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, F,Yang, XX,Hu, WG,et al. Purification and characterization of trichokirin-S1, a novel ribosome-inactivating peptide from seeds of Trichosanthes kirilowii[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(9):841-846. |
| APA | Li, F,Yang, XX,Hu, WG,Xia, HC,Li, Z,&Zhang, ZC.(2003).Purification and characterization of trichokirin-S1, a novel ribosome-inactivating peptide from seeds of Trichosanthes kirilowii.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(9),841-846. |
| MLA | Li, F,et al."Purification and characterization of trichokirin-S1, a novel ribosome-inactivating peptide from seeds of Trichosanthes kirilowii".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.9(2003):841-846. |
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