Purification and partial characterization of luffin P1, a peptide with translational inhibitory activity and trypsin inhibitory activity, from seeds of Luffa cylindrica
文献类型：期刊论文
| 作者 | Li, F; Xia, HC; Yang, XX; Hu, WG; Li, Z; Zhang, ZC |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:9页码:847-852 |
| 关键词 | seeds of Luffa cylindrical luffin P1 translational inhibitory activity trypsin inhibitory activity |
| 通讯作者 | Zhang, ZC (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Proteom, Shanghai 200031, Peoples R China., |
| 英文摘要 | A peptide, luffin P1, from seeds of Luffa cylindrica, was purified by ammonia sulfate precipitation, CM-52 ion exchange chromatography, Blue-gel affinity chromatography and FPLC Mono S ion exchange chromatography. Its molecular weight was 5226.5 as determined by MALDI-TOF-MS analysis. The sequence of N-terminal 11 amino acids of luffin P was identical with the partial N-terminal sequence (from G3 to R13) of 6.5K Arg/Glu rich peptide, which was also isolated from the seeds of Luffa cylindrica. Besides, luffin P1 had a very high homology with a trypsin inhibitor, named C2 peptide, from pumpkin seeds. Interestingly, the purified luffin P1 not only showed a strong inhibitory activity on protein synthesis in rabbit reticulocyte lysate cell-free translation system with IC50 of 0.6 nmol/L, but also had trypsin inhibitory activity with IC50 of 22 mumol/L. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | RIBOSOME-INACTIVATING PROTEIN ; MECHANISM ; INTEGRASE ; RNA |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000185493600012 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2344]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, F,Xia, HC,Yang, XX,et al. Purification and partial characterization of luffin P1, a peptide with translational inhibitory activity and trypsin inhibitory activity, from seeds of Luffa cylindrica[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(9):847-852. |
| APA | Li, F,Xia, HC,Yang, XX,Hu, WG,Li, Z,&Zhang, ZC.(2003).Purification and partial characterization of luffin P1, a peptide with translational inhibitory activity and trypsin inhibitory activity, from seeds of Luffa cylindrica.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(9),847-852. |
| MLA | Li, F,et al."Purification and partial characterization of luffin P1, a peptide with translational inhibitory activity and trypsin inhibitory activity, from seeds of Luffa cylindrica".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.9(2003):847-852. |
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