Gene therapy of experimental autoimmune thyroiditis mice by in vivo administration of plasmid DNA coding for human interleukin-10
文献类型：期刊论文
| 作者 | Zhang, ZL; Lin, B; Yu, LY; Shen, SX; Zhu, LH; Wang, WP; Guo, LH |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 24期号:9页码:885-890 |
| 关键词 | interleukin-10 gene therapy thyroiditis autoimmune diseases |
| 通讯作者 | Guo, LH (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,lhguo@sunm.shcnc.ac.cn |
| 英文摘要 | AIM: To investigate the effect of interleukin-10 (IL-10) gene on experimental autoimmune thyroiditis mice. METHODS: Mice were immunized to induce autoimmune thyroiditis with porcine thyroglobulin (pTg), and thyroids of mice were injected with IL-10 DNA. On d 28 after immunization with pTg, mRNA expression of IL-10 in thyroid glands was detected and thyroid specimens were histopathological studied. RESULTS: The mRNA expression of IL-10 was detected in thyroid glands on d 7 and 14 after injection of IL-10 plasmid DNA or on COS-7 cells 48 h after IL-10 plasmid DNA transfection. In addition, hIL-10 levels in culture media significantly increased 48 h and 72 h after IL-10 plasmid DNA transfection. Infiltration index of lymphocytes (1.1 +/- 0.4) in thyroids of IL-10-treated mice was significantly lower than that of pcDNA3-null-treated mice (2.2 +/- 0.5) (P<0.01). Compared with pcDNA3-null control mice, IL-10-treated mice had lower levels of serum IFN-gamma (P<0.01). CONCLUSION: The direct injection of DNA expression vectors encoding IL-10 into thyroid significantly inhibited development of lymphocytic infiltration of thyroid of autoimmune thyroiditis mice, and alleviated the progression of this disease. |
| 学科主题 | Chemistry; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 关键词[WOS] | LIPOSOME COMPLEXES ; FAS LIGAND ; T-CELLS ; EXPRESSION ; TOXICITY ; DISEASE ; EAT ; DELIVERY ; GAMMA ; IL-10 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000185357500008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2346]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhang, ZL,Lin, B,Yu, LY,et al. Gene therapy of experimental autoimmune thyroiditis mice by in vivo administration of plasmid DNA coding for human interleukin-10[J]. ACTA PHARMACOLOGICA SINICA,2003,24(9):885-890. |
| APA | Zhang, ZL.,Lin, B.,Yu, LY.,Shen, SX.,Zhu, LH.,...&Guo, LH.(2003).Gene therapy of experimental autoimmune thyroiditis mice by in vivo administration of plasmid DNA coding for human interleukin-10.ACTA PHARMACOLOGICA SINICA,24(9),885-890. |
| MLA | Zhang, ZL,et al."Gene therapy of experimental autoimmune thyroiditis mice by in vivo administration of plasmid DNA coding for human interleukin-10".ACTA PHARMACOLOGICA SINICA 24.9(2003):885-890. |
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