中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Effects of green tea polyphenols on dopamine uptake and on MPP+-induced dopamine neuron injury

文献类型:期刊论文

作者Pan, TH; Fei, J; Zhou, XD; Jankovic, J; Le, WD
刊名LIFE SCIENCES
出版日期2003
卷号72期号:9页码:1073-1083
关键词Parkinson's disease dopamine transporter green tea polyphenols 1-methyl-4-phenylpyridinium neuroprotection
通讯作者Le, WD (reprint author), Baylor Coll Med, Dept Neurol, 1 Baylor Plaza, Houston, TX 77030 USA.,
英文摘要As antioxidants, polyphenols are considered to be potentially useful in preventing chronic diseases in man, including Parkinson's disease (PD), a disease involving dopamine (DA) neurons. Our studies have demonstrated that polyphenols extracted from green tea (GT) can inhibit the uptake of H-3-dopamine (H-3-DA) and 1-methyl-4-phenylpyridinium (MPP+) by DA transporters (DAT) and partially protect embryonic rat mesencephalic dopaminergic (DAergic) neurons from MPP+-induced injury. The inhibitory effects of GT polyphenols on H-3-DA uptake were determined in DAT-pCDNA3-traiisfected Chinese Hamster Ovary (DAT-CHO) cells and in striatal synaptosomes of C57BL/6 mice in vitro and in vivo. The inhibitory effects on H-3-MPP+ uptake were determined in primary cultures of embryonic rat mesencephalic DAergic cells. Inhibition of uptake for both H-3-DA and H-3-MPP+ was dose-dependent in the presence of polyphenols. Incubation with 50 muM MPP+ resulted in a significant loss of tyrosine-hydroxylase (TH)-positive cells in the primary embryonic mesencephalic cultures, while pretreatment with polyphenols (10 to 30 mug/ml) or mazindol (10 muM), a classical DAT inhibitor, significantly attenuated MPP+-induced loss of TH-positive cells. These results suggest that GT polyphenols have inhibitory effects on DAT, through which they block MPP+ uptake and protect DAergic neurons against MPP+-induced injury. (C) 2002 Elsevier Science Inc. All rights reserved.
学科主题Research & Experimental Medicine; Pharmacology & Pharmacy
类目[WOS]Medicine, Research & Experimental ; Pharmacology & Pharmacy
关键词[WOS]PARKINSONS-DISEASE ; BLACK TEA ; TRANSPORTER ; RAT ; COCAINE ; GENE ; MPTP ; (-)-EPIGALLOCATECHIN-3-GALLATE ; NEUROTOXIN ; EXPRESSION
收录类别SCI
语种英语
WOS记录号WOS:000180518400009
版本出版稿
源URL[http://202.127.25.143/handle/331003/2350]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714
Pan, TH,Fei, J,Zhou, XD,et al. Effects of green tea polyphenols on dopamine uptake and on MPP+-induced dopamine neuron injury[J]. LIFE SCIENCES,2003,72(9):1073-1083.
APA Pan, TH,Fei, J,Zhou, XD,Jankovic, J,&Le, WD.(2003).Effects of green tea polyphenols on dopamine uptake and on MPP+-induced dopamine neuron injury.LIFE SCIENCES,72(9),1073-1083.
MLA Pan, TH,et al."Effects of green tea polyphenols on dopamine uptake and on MPP+-induced dopamine neuron injury".LIFE SCIENCES 72.9(2003):1073-1083.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。