Effects of green tea polyphenols on dopamine uptake and on MPP+-induced dopamine neuron injury
文献类型：期刊论文
| 作者 | Pan, TH; Fei, J; Zhou, XD; Jankovic, J; Le, WD |
| 刊名 | LIFE SCIENCES
 |
| 出版日期 | 2003 |
| 卷号 | 72期号:9页码:1073-1083 |
| 关键词 | Parkinson's disease dopamine transporter green tea polyphenols 1-methyl-4-phenylpyridinium neuroprotection |
| 通讯作者 | Le, WD (reprint author), Baylor Coll Med, Dept Neurol, 1 Baylor Plaza, Houston, TX 77030 USA., |
| 英文摘要 | As antioxidants, polyphenols are considered to be potentially useful in preventing chronic diseases in man, including Parkinson's disease (PD), a disease involving dopamine (DA) neurons. Our studies have demonstrated that polyphenols extracted from green tea (GT) can inhibit the uptake of H-3-dopamine (H-3-DA) and 1-methyl-4-phenylpyridinium (MPP+) by DA transporters (DAT) and partially protect embryonic rat mesencephalic dopaminergic (DAergic) neurons from MPP+-induced injury. The inhibitory effects of GT polyphenols on H-3-DA uptake were determined in DAT-pCDNA3-traiisfected Chinese Hamster Ovary (DAT-CHO) cells and in striatal synaptosomes of C57BL/6 mice in vitro and in vivo. The inhibitory effects on H-3-MPP+ uptake were determined in primary cultures of embryonic rat mesencephalic DAergic cells. Inhibition of uptake for both H-3-DA and H-3-MPP+ was dose-dependent in the presence of polyphenols. Incubation with 50 muM MPP+ resulted in a significant loss of tyrosine-hydroxylase (TH)-positive cells in the primary embryonic mesencephalic cultures, while pretreatment with polyphenols (10 to 30 mug/ml) or mazindol (10 muM), a classical DAT inhibitor, significantly attenuated MPP+-induced loss of TH-positive cells. These results suggest that GT polyphenols have inhibitory effects on DAT, through which they block MPP+ uptake and protect DAergic neurons against MPP+-induced injury. (C) 2002 Elsevier Science Inc. All rights reserved. |
| 学科主题 | Research & Experimental Medicine; Pharmacology & Pharmacy |
| 类目[WOS] | Medicine, Research & Experimental ; Pharmacology & Pharmacy |
| 关键词[WOS] | PARKINSONS-DISEASE ; BLACK TEA ; TRANSPORTER ; RAT ; COCAINE ; GENE ; MPTP ; (-)-EPIGALLOCATECHIN-3-GALLATE ; NEUROTOXIN ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000180518400009 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2350]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Pan, TH,Fei, J,Zhou, XD,et al. Effects of green tea polyphenols on dopamine uptake and on MPP+-induced dopamine neuron injury[J]. LIFE SCIENCES,2003,72(9):1073-1083. |
| APA | Pan, TH,Fei, J,Zhou, XD,Jankovic, J,&Le, WD.(2003).Effects of green tea polyphenols on dopamine uptake and on MPP+-induced dopamine neuron injury.LIFE SCIENCES,72(9),1073-1083. |
| MLA | Pan, TH,et al."Effects of green tea polyphenols on dopamine uptake and on MPP+-induced dopamine neuron injury".LIFE SCIENCES 72.9(2003):1073-1083. |
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