Isolation of a multi-functional endogenous cellulase gene from mollusc, Ampullaria crossean
文献类型：期刊论文
| 作者 | Wang, J; Ding, M; Li, YH; Chen, QX; Xu, GJ; Zhao, FK |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:10页码:941-946 |
| 关键词 | multi-functional cellulase EGX gene isolation Ampullaria crossean |
| 通讯作者 | Zhao, FK (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Key Lab Proteom, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China., |
| 英文摘要 | The cellulase genes of some animals, most coding for endo-beta-1,4-glucanases, were found and cloned. There has been no reports about genes encoding exo-beta-1, 4-glucanase or endo-beta-1, 4-xylanase from animal. Here we cloned the cDNA of a cellulase designated as EGX from mollusc, Antpullaria crossean, and expressed it in Pichia pastoris for the first time. The cellulase EGX is a multifunctional cellulase with the activities of exo-beta-1,4-glucanase, endo-beta-1,4-glucanase and endo-beta-1,4xylanase. The opening reading frame of EGX cDNA is 1185 by and encodes 395 amino acids. The EGX gene can also be amplificated from the genomic DNA by PCR, which verged the endogenous origin of this gene. This EGX gene was the first multi-functional cellulase gene that was directly isolated from animals. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | CELLULOMONAS-FIMI ; TERMITE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000186075100013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2355]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, J,Ding, M,Li, YH,et al. Isolation of a multi-functional endogenous cellulase gene from mollusc, Ampullaria crossean[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(10):941-946. |
| APA | Wang, J,Ding, M,Li, YH,Chen, QX,Xu, GJ,&Zhao, FK.(2003).Isolation of a multi-functional endogenous cellulase gene from mollusc, Ampullaria crossean.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(10),941-946. |
| MLA | Wang, J,et al."Isolation of a multi-functional endogenous cellulase gene from mollusc, Ampullaria crossean".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.10(2003):941-946. |
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