中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Isolation of a multi-functional endogenous cellulase gene from mollusc, Ampullaria crossean

文献类型:期刊论文

作者Wang, J; Ding, M; Li, YH; Chen, QX; Xu, GJ; Zhao, FK
刊名ACTA BIOCHIMICA ET BIOPHYSICA SINICA
出版日期2003
卷号35期号:10页码:941-946
关键词multi-functional cellulase EGX gene isolation Ampullaria crossean
通讯作者Zhao, FK (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Key Lab Proteom, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China.,
英文摘要The cellulase genes of some animals, most coding for endo-beta-1,4-glucanases, were found and cloned. There has been no reports about genes encoding exo-beta-1, 4-glucanase or endo-beta-1, 4-xylanase from animal. Here we cloned the cDNA of a cellulase designated as EGX from mollusc, Antpullaria crossean, and expressed it in Pichia pastoris for the first time. The cellulase EGX is a multifunctional cellulase with the activities of exo-beta-1,4-glucanase, endo-beta-1,4-glucanase and endo-beta-1,4xylanase. The opening reading frame of EGX cDNA is 1185 by and encodes 395 amino acids. The EGX gene can also be amplificated from the genomic DNA by PCR, which verged the endogenous origin of this gene. This EGX gene was the first multi-functional cellulase gene that was directly isolated from animals.
学科主题Biochemistry & Molecular Biology; Biophysics
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]CELLULOMONAS-FIMI ; TERMITE
收录类别SCI
语种英语
WOS记录号WOS:000186075100013
版本出版稿
源URL[http://202.127.25.143/handle/331003/2355]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714
Wang, J,Ding, M,Li, YH,et al. Isolation of a multi-functional endogenous cellulase gene from mollusc, Ampullaria crossean[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(10):941-946.
APA Wang, J,Ding, M,Li, YH,Chen, QX,Xu, GJ,&Zhao, FK.(2003).Isolation of a multi-functional endogenous cellulase gene from mollusc, Ampullaria crossean.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(10),941-946.
MLA Wang, J,et al."Isolation of a multi-functional endogenous cellulase gene from mollusc, Ampullaria crossean".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.10(2003):941-946.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。