Long term gene therapy of Parkinson's disease using immortalized rat glial cell line with tyrosine hydroxylase gene
文献类型：期刊论文
| 作者 | Zhuo, M; Xu, DH; Cao, L; Xu, LF; Yu, FR; Zheng, ZC; Liu, XY |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 35期号:12页码:1066-1071 |
| 关键词 | Parkinson's disease gene therapy glial cell SV40 large T-antigen tyrosine hydroxylase |
| 通讯作者 | Liu, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | Glial cell is an ideal vehicle for gene therapy of brain diseases. However, there are many limits in using primary glial cells. Therefore, an immortalized rat glial cell line (RGLT) was established by SV40 large T-antigen (LTag) gene from the primary rat fetal glial cells. The RGLT cell was shown to be non-tumorigenic after transplantation to nude mice (up to 4 weeks) and rat striatum (up to 18 months). Rat tyrosine hydroxylase (TH) gene was transfected into RGLT cell to obtain RGLT-TH cell.. The TH immunohistochemical staining and HPLC-ECD analysis demonstrated the TH expression and dopamine (DA) production in RGLT-TH cells in vitro. When implanting RGLT-TH cells into the striatum. of 6-hydroxydopamine (6-OHDA) lesioned hemiparkinsonism model rats, TH immunohistochemical staining showed the TH presence in striatum and HPLC-ECD analysis held at 6 months after cell implantation showed an increase of DA content in striatum. The asymmetric rotation of rats receiving RGLT-TH cells was reduced by 50% - 60% and this reduction persisted stably at least for 18 months. These results suggest that the immortalized glial cell line could serve as an ideal vehicle for therapeutic gene delivery system to achieve a long-term gene therapy of neurodegenerative diseases. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | AMINO-ACID DECARBOXYLASE ; T-ANTIGEN GENE ; PRODUCE L-DOPA ; MODEL ; ASTROCYTES ; STRIATUM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000187534300003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2365]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Zhuo, M,Xu, DH,Cao, L,et al. Long term gene therapy of Parkinson's disease using immortalized rat glial cell line with tyrosine hydroxylase gene[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(12):1066-1071. |
| APA | Zhuo, M.,Xu, DH.,Cao, L.,Xu, LF.,Yu, FR.,...&Liu, XY.(2003).Long term gene therapy of Parkinson's disease using immortalized rat glial cell line with tyrosine hydroxylase gene.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(12),1066-1071. |
| MLA | Zhuo, M,et al."Long term gene therapy of Parkinson's disease using immortalized rat glial cell line with tyrosine hydroxylase gene".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.12(2003):1066-1071. |
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