CMV-hFasL transgenic mice are sensitive to low doses of streptozotocin-induced type I diabetes mellitus
文献类型：期刊论文
| 作者 | Lin, B; Zhang, ZL; Yu, LY; Guo, LH |
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 24期号:12页码:1199-1204 |
| 关键词 | transgenic mice Fas Fas ligand type I diabetes mellitus streptozotocin apoptosis interleukin-1 tumor necrosis factor |
| 通讯作者 | Zhang, ZL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China.,lhguo@sunm.shcnc.ac.cn |
| 英文摘要 | AIM: To investigate the role of Fas-FasL pathway in the pathogenesis of streptozotocin (STZ)-induced type I diabetes mellitus. METHODS: Low dose injections of STZ were used to induce type I diabetes mellitus in the CMV-hFasL transgenic mice. Blood glucose concentration was measured with Glucotrand Plus blood glucose test strips. Expression of hFasL was detected by RT-PCR and Western blotting. The severity of insulitis was determined by histological examination. Expressions of IL-1beta and TNF-alpha mRNA in the pancreas were detected by semi-quantitative RT-PCR analysis. Fas expression in apoptotic RIN-5F cells was also confirmed by RT-PCR in vitro. RESULTS: hFasL was expressed in the islets of CMV-hFasL transgenic mice. The transgenic mice were sensitive to diabetic induction than the control WT mice. IL-1beta and TNF-alpha expressions in the pancreas of CMV-hFasL transgenic mice were far more than that in WT mice. We also found STZ and IL-1beta could both induce higher expression of Fas in RIN-5F. The combining of Fas-FasL could lead to the apoptosis of beta cells in the CMV-hFasL transgenic mice. CONCLUSION: Fas-FasL interaction plays a significant role in the pathogenic mechanism of type I diabetes mellitus. |
| 学科主题 | Chemistry; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 关键词[WOS] | BETA-CELL DESTRUCTION ; FAS LIGAND ; NOD MICE ; APOPTOSIS ; MOUSE ; DEATH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000187218600004 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2367]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Lin, B,Zhang, ZL,Yu, LY,et al. CMV-hFasL transgenic mice are sensitive to low doses of streptozotocin-induced type I diabetes mellitus[J]. ACTA PHARMACOLOGICA SINICA,2003,24(12):1199-1204. |
| APA | Lin, B,Zhang, ZL,Yu, LY,&Guo, LH.(2003).CMV-hFasL transgenic mice are sensitive to low doses of streptozotocin-induced type I diabetes mellitus.ACTA PHARMACOLOGICA SINICA,24(12),1199-1204. |
| MLA | Lin, B,et al."CMV-hFasL transgenic mice are sensitive to low doses of streptozotocin-induced type I diabetes mellitus".ACTA PHARMACOLOGICA SINICA 24.12(2003):1199-1204. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。