Bacterial virus phi29 pRNA as a hammerhead ribozyme escort to destroy hepatitis B virus
文献类型：期刊论文
| 作者 | Hoeprich, S; Zhou, Q; Guo, S; Shu, D; Qi, G; Wang, Y; Guo, P |
| 刊名 | GENE THERAPY
 |
| 出版日期 | 2003 |
| 卷号 | 10期号:15页码:1258-1267 |
| 关键词 | pRNA of bacteriophage phi29 hammerhead fibozyme gene therapy nanomotor DNA packaging hexamer |
| 通讯作者 | Guo, P (reprint author), Purdue Univ, Purdue Canc Res Ctr, Dept Pathobiol, B-36 Hansen Life Sci Res Bldg, W Lafayette, IN 47907 USA., |
| 英文摘要 | The DNA-packaging pRNA of bacterial virus phi29, which forms dimers and then hexamers, contains two independent tightly self-folded domains. Circularly permuted pRNAs were constructed without impacting pRNA folding. Connecting the pRNA 5'/3' ends with variable sequences did not disturb its folding and function. These unique features, which help prevent two common problems - exonuclease degradation and misfolding in the cell, make pRNA an ideal vector to carry therapeutic RNAs. A pRNA-based vector was designed to carry hammerhead ribozymes that cleave the hepatitis B virus (HBV) polyA signal. The chimeric HBV-targeting ribozyme was connected to the pRNA 5'/3' ends as circularly permuted pRNA. Two cis-cleaving ribozymes were used to flank and process the chimeric ribozyme. The hammerhead ribozyme including its two arms for HBV targeting was able to fold correctly while escorted by the pRNA. The chimeric ribozyme cleaved the polyA signal of HBV mRNA in vitro almost completely. Cell culture studies showed that the chimeric ribozyme was able to enhance the inhibition of HBV replication when compared with the ribozyme not escorted by pRNA, as demonstrated by Northern blot and e-antigen assays. pRNA could also carry another hammerhead ribozyme to cleave other RNA substrate. These findings suggest that pRNA can be used as a vector for imparting stability to ribozymes, antisense, and other therapeutic RNA molecules in vivo. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental |
| 关键词[WOS] | DNA PACKAGING MOTOR ; PHAGE PHI-29 ; BACTERIOPHAGE-PHI-29 DNA ; CHEMICAL MODIFICATION ; VIRAL-RNA ; SEQUENCE REQUIREMENT ; RIBONUCLEASE-P ; HEXAMERIC RNA ; PROHEAD RNA ; IN-VITRO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000184207000008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2375]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Hoeprich, S,Zhou, Q,Guo, S,et al. Bacterial virus phi29 pRNA as a hammerhead ribozyme escort to destroy hepatitis B virus[J]. GENE THERAPY,2003,10(15):1258-1267. |
| APA | Hoeprich, S.,Zhou, Q.,Guo, S.,Shu, D.,Qi, G.,...&Guo, P.(2003).Bacterial virus phi29 pRNA as a hammerhead ribozyme escort to destroy hepatitis B virus.GENE THERAPY,10(15),1258-1267. |
| MLA | Hoeprich, S,et al."Bacterial virus phi29 pRNA as a hammerhead ribozyme escort to destroy hepatitis B virus".GENE THERAPY 10.15(2003):1258-1267. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。