A novel conotoxin from Conus betulinus, kappa-BtX, unique in cysteine pattern and in function as a specific BK channel modulator
文献类型：期刊论文
| 作者 | Fan, CX; Chen, XK; Zhang, C; Wang, LX; Duan, KL; He, LL; Cao, Y; Liu, SY; Zhong, MN; Ulens, C |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2003 |
| 卷号 | 278期号:15页码:12624-12633 |
| 通讯作者 | Zhou, Z (reprint author), Res Inst Pharmaceut Chem, Beijing 102205, Peoples R China., |
| 英文摘要 | A novel conotoxin, kappa-conotoxin (kappa-BtX), has been purified and characterized from the venom of a worm-hunting cone snail, Conus betulinus. The toxin, with four disulfide bonds, shares no sequence homology with any other conotoxins. Based on a partial amino acid sequence, its cDNA was cloned and sequenced. The deduced sequence consists of a 26-residue putative signal peptide, a 31-residue mature toxin, and a 13-residue extra peptide at the C terminus. The extra peptide is cleaved off by proteinase post-processing. All three Glu residues are gamma-carboxylated, one of the two Pro residues is hydroxylated at position 27, and its C-terminal residue is Pro-amidated. The monoisotopic mass of the toxin is 3569.0 Da. Electrophysiological experiments show that: 1) among voltage-gated channels;, K-BtX is a specific modulator of K+ channels; 2) among the K channels, kappa-BtX specifically up-modulates the Ca2+- and voltage-sensitive BK channels (252 47%); 3) its EC50 is 0.7 nm with a single binding site (Hill = 0.88); 4) the time constant of wash-out is 8.3 s; and 5) kappa-BtX has no effect on single channel conductance, but increases the open probability of BK channels. It is concluded that kappa-BtX is a novel specific biotoxin against BK channels. |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | ADRENAL CHROMAFFIN CELLS ; CA2+-ACTIVATED K+ CHANNELS ; POTASSIUM CHANNELS ; MOLECULAR-BASIS ; VENOM PEPTIDES ; VOLTAGE ; CHARYBDOTOXIN ; INACTIVATION ; CONDUCTANCE ; DIVERSITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000182189500007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2376]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Fan, CX,Chen, XK,Zhang, C,et al. A novel conotoxin from Conus betulinus, kappa-BtX, unique in cysteine pattern and in function as a specific BK channel modulator[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2003,278(15):12624-12633. |
| APA | Fan, CX.,Chen, XK.,Zhang, C.,Wang, LX.,Duan, KL.,...&Zhou, Z.(2003).A novel conotoxin from Conus betulinus, kappa-BtX, unique in cysteine pattern and in function as a specific BK channel modulator.JOURNAL OF BIOLOGICAL CHEMISTRY,278(15),12624-12633. |
| MLA | Fan, CX,et al."A novel conotoxin from Conus betulinus, kappa-BtX, unique in cysteine pattern and in function as a specific BK channel modulator".JOURNAL OF BIOLOGICAL CHEMISTRY 278.15(2003):12624-12633. |
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