Adenovirus-mediated interleukin-2 gene therapy of nociception
文献类型：期刊论文
| 作者 | Yao, MZ; Gu, JF; Wang, JH; Sun, LY; Liu, H; Liu, XY |
| 刊名 | GENE THERAPY
 |
| 出版日期 | 2003 |
| 卷号 | 10期号:16页码:1392-1399 |
| 关键词 | adenovirus interleukin-2 gene therapy intrathecal delivery nociception antinociceptive effect |
| 通讯作者 | Liu, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | The effect of adenovirus-mediated interleukin-2 (IL-2) gene on rat basal nociceptive response and chronic neuropathic pain was explored. The paw withdrawal latency induced by radiant heat was used to evaluate the antinociceptive effect of adenovirus type 5 (Ad5) and Ad5-IL-2. The results showed that intrathecal delivery of Ad5-IL-2 exhibited obvious antinociceptive effects on basal nociceptive response and chronic neuropathic pain, which were maintained for 3 and 4 weeks, respectively. This suggested that the antinociceptive effect of Ad5-IL-2 on chronic neuropathic pain was greater than its effect on basal nociceptive response. Human IL-2 mRNA was detected by in situ hybridization in the spinal pia mater and parenchyma of the lumbar, sacral, thoracic and cervical regions, and gray matter had higher level of IL-2 expression than white matter. These data demonstrated that the IL-2 gene was transfected into spinal cord regions relevant to pain modulation. The expressed IL-2 protein profile in spinal cord detected by enzyme-linked immunosorbent assay coincided almost exactly with its antinociceptive effect. This supported the hypothesis that the therapeutic effect of IL-2 gene was related to IL-2 protein expression. The study indicates that intrathecal delivery of adenovirus-mediated IL-2 gene has a relatively long antinociceptive effect. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental |
| 关键词[WOS] | SPINAL SUBARACHNOID SPACE ; CENTRAL-NERVOUS-SYSTEM ; SUBSTANCE-P ; OPIOID RECEPTOR ; RAT ; PAIN ; IL-2 ; NEURONS ; IMMUNOREACTIVITIES ; ANTINOCICEPTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000184427700014 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2377]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yao, MZ,Gu, JF,Wang, JH,et al. Adenovirus-mediated interleukin-2 gene therapy of nociception[J]. GENE THERAPY,2003,10(16):1392-1399. |
| APA | Yao, MZ,Gu, JF,Wang, JH,Sun, LY,Liu, H,&Liu, XY.(2003).Adenovirus-mediated interleukin-2 gene therapy of nociception.GENE THERAPY,10(16),1392-1399. |
| MLA | Yao, MZ,et al."Adenovirus-mediated interleukin-2 gene therapy of nociception".GENE THERAPY 10.16(2003):1392-1399. |
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