Analysis of chicken and turkey ovalburnins by microchip electrophoresis combined with exoglycosidase digestion
文献类型：期刊论文
| 作者 | Mao, XL; Wang, K; Du, YG; Lin, BC |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2003 |
| 卷号 | 24期号:18页码:3273-3278 |
| 关键词 | chicken ovalbumin glycopeptides microchip electrophoresis miniaturization peptide mapping Turkey ovalburnin |
| 通讯作者 | Lin, BC (reprint author), Chinese Acad Sci, Dalian Inst Chem Phys, 457 Zhongshan Rd, Dalian 116023, Peoples R China., |
| 英文摘要 | The polypeptide and carbohydrate patterns of two glycoproteins, chicken ovalbumin (CO) and turkey ovalbumin (TO), were analyzed by microchip electrophoresis (ME), following digestion with proteases and exoglycosidases. Glycopeptides derived from ovalbumin were obtained by digestion with Pronase, followed by dialysis, and then separated by ME. Using CO as model, the method was developed to deduce the structure of glycans from glycoproteins by comparing the electropherograms of glycopeptides with and without digestion of exolycosidases. Applying the same approach, the structure of oligosaccharides linked to TO was determined. TO was found to contain high-mannose type oligosaccharides and oligosaccharides with terminal N-acetylglucosamine residues. The complete primary analysis of CO and TO by ME described in this paper provides a basis for an analysis of glycoproteins with an integrated microfluidic chip. |
| 学科主题 | Biochemistry & Molecular Biology; Chemistry |
| 类目[WOS] | Biochemical Research Methods ; Chemistry, Analytical |
| 关键词[WOS] | CAPILLARY-ELECTROPHORESIS ; MASS-SPECTROMETRY ; OVALBUMIN ; GLYCOSYLATION ; GLYCOPROTEINS ; OLIGOSACCHARIDES ; PROTEOMICS ; SEQUENCE ; SYSTEM ; CHIP |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000185897100026 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2382]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Mao, XL,Wang, K,Du, YG,et al. Analysis of chicken and turkey ovalburnins by microchip electrophoresis combined with exoglycosidase digestion[J]. ELECTROPHORESIS,2003,24(18):3273-3278. |
| APA | Mao, XL,Wang, K,Du, YG,&Lin, BC.(2003).Analysis of chicken and turkey ovalburnins by microchip electrophoresis combined with exoglycosidase digestion.ELECTROPHORESIS,24(18),3273-3278. |
| MLA | Mao, XL,et al."Analysis of chicken and turkey ovalburnins by microchip electrophoresis combined with exoglycosidase digestion".ELECTROPHORESIS 24.18(2003):3273-3278. |
入库方式： OAI收割
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