GBP binds kinesin light chain and translocates during cortical rotation in Xenopus eggs
文献类型：期刊论文
| 作者 | Weaver, C; Farr, GH; Pan, WJ; Rowning, BA; Wang, JY; Mao, JH; Wu, DQ; Li, L; Larabell, CA; Kimelman, D |
| 刊名 | DEVELOPMENT
 |
| 出版日期 | 2003 |
| 卷号 | 130期号:22页码:5425-5436 |
| 关键词 | Frat Wnt pathway axis specification cortical rotation microtubules |
| 通讯作者 | Kimelman, D (reprint author), Univ Washington, Dept Biochem, Seattle, WA 98195 USA., |
| 英文摘要 | In Xenopus, axis development is initiated by dorsally elevated levels of cytoplasmic beta-catenin, an intracellular factor regulated by GSK3 kinase activity. Upon fertilization, factors that increase beta-catenin stability are translocated to the prospective dorsal side of the embryo in a microtubule-dependent process. However, neither the identity of these factors nor the mechanism of their movement is understood. Here, we show that the GSK3 inhibitory protein GBP/Frat binds kinesin light chain (KLC), a component of the microtubule motor kinesin. Upon egg activation, GBP-GFP and KLC-GFP form particles and exhibit directed translocation. KLC, through a previously uncharacterized conserved domain, binds a region of GBP that is required for GBP translocation and for GSK3 binding, and competes with GSK3 for GBP. We propose a model in which conventional kinesin transports a GBP-containing complex to the future dorsal side, where GBP dissociates and contributes to the local stabilization of beta-catenin by binding and inhibiting GSK3. |
| 学科主题 | Developmental Biology |
| 类目[WOS] | Developmental Biology |
| 关键词[WOS] | GLYCOGEN-SYNTHASE KINASE-3 ; AXIS-INDUCING ACTIVITY ; BETA-CATENIN ; SIGNALING PATHWAY ; AXONAL-TRANSPORT ; EMBRYONIC AXIS ; VENTRAL AXIS ; WNT PATHWAY ; CELL-CYCLE ; I-EPSILON |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000187399800013 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2386]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Weaver, C,Farr, GH,Pan, WJ,et al. GBP binds kinesin light chain and translocates during cortical rotation in Xenopus eggs[J]. DEVELOPMENT,2003,130(22):5425-5436. |
| APA | Weaver, C.,Farr, GH.,Pan, WJ.,Rowning, BA.,Wang, JY.,...&Kimelman, D.(2003).GBP binds kinesin light chain and translocates during cortical rotation in Xenopus eggs.DEVELOPMENT,130(22),5425-5436. |
| MLA | Weaver, C,et al."GBP binds kinesin light chain and translocates during cortical rotation in Xenopus eggs".DEVELOPMENT 130.22(2003):5425-5436. |
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