A novel method for polypeptide design to prepare specific antibody of the peptide and applied to immunoassay
文献类型：期刊论文
| 作者 | Liu, ZH; Li, MY; Cui, DF |
| 刊名 | JOURNAL OF IMMUNOLOGICAL METHODS
 |
| 出版日期 | 2003 |
| 卷号 | 281期号:1页码:17-25 |
| 关键词 | polypeptide synthesis specific antibody competitive ELISA OGP penetratin |
| 通讯作者 | Cui, DF (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Peptide was polymerized by a novel method: compound containing double bond between two carbons such as acdryloyl chloride was introduced into peptide during peptide synthesis, then it was transformed to a polymer which has a poly propionyl core matrix with peptide branches by radical polymerization either after being cleaved from resin or before being cleaved from resin according to the peptide physical-chemical character. According to this design, the macromolecules with average MW about 40 kD for poly-Osteogenic Growth Peptide (poly-OGP), 25 KD for poly-penetratin could be produced. Poly-OGP was further applied for antibody preparation and immunoassays. Immunizing New Zealand rabbits, we obtained the antiserum with titer of 2.5 x 10(4), examined by enzyme-linked immunosorbent assay (ELISA), without cross-reaction with bovine serum albumin (BSA), while the antiserum produced by using BSA as peptide carrier strongly reacted with BSA (with titer of more than 50 x 10(4) for BSA). Based on competitive ELISA, the anti-poly-OGP antibody showed much better immunoreactive sensitivity than anti-BSA-OGP antibody by comparing their IC50 toward OGP. The antigen determinant of OGP and the OGP content in the serum of mice, determined by anti-poly-OGP antibody, showed that the anti-polypeptide antibody can be used as tools for inummoassay. Thus, the polypeptide system is not only a new approach for preparing synthetic peptide antibody for immunoassays but also provided the prospect for preparing synthetic peptide-based vaccine. (C) 2003 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Immunology |
| 类目[WOS] | Biochemical Research Methods ; Immunology |
| 关键词[WOS] | OSTEOGENIC GROWTH PEPTIDE ; PROTEIN ; VACCINE ; OGP |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000186487500003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2393]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Liu, ZH,Li, MY,Cui, DF. A novel method for polypeptide design to prepare specific antibody of the peptide and applied to immunoassay[J]. JOURNAL OF IMMUNOLOGICAL METHODS,2003,281(1):17-25. |
| APA | Liu, ZH,Li, MY,&Cui, DF.(2003).A novel method for polypeptide design to prepare specific antibody of the peptide and applied to immunoassay.JOURNAL OF IMMUNOLOGICAL METHODS,281(1),17-25. |
| MLA | Liu, ZH,et al."A novel method for polypeptide design to prepare specific antibody of the peptide and applied to immunoassay".JOURNAL OF IMMUNOLOGICAL METHODS 281.1(2003):17-25. |
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