Lef/Tcf-dependent Wnt/beta-catenin signaling during Xenopus axis specification
文献类型：期刊论文
| 作者 | Geng, X; Xiao, L; Lin, FG; Hu, RY; Wang, JH; Rupp, RAW; Ding, XY |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2003 |
| 卷号 | 547期号:1页码:42010 |
| 关键词 | axis specification Wnt beta-catenin Lef-1 Tcf restriction enzyme-mediated integration transgenesis Xenopus |
| 通讯作者 | Ding, XY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Lab Stem Cell Biol,Lab Mol & Cell Biol, Yue Yang Rd 320, Shanghai 200031, Peoples R China., |
| 英文摘要 | Though the Wnt/beta-catenin signaling pathway is known to play key roles during Xenopus axis specification, whether it signals exclusively through Lef/Tcf transcription factors in this process remains unclear. To investigate this issue, we generated transgenic frog embryos expressing green fluorescent protein (GFP) driven by a Lef/Tcf-dependent and Wnt/beta-catenin-responsive promoter. This promoter is highly sensitive and even detects maternal beta-catenin activity prior to the large-scale transcription of zygotic genes. Unexpectedly, GFP expression was observed only in some, but not all, known Wnt/beta-catenin-positive territories in Xenopus early development. Furthermore, ubiquitous expression of dominant Lef-1 protein variants from transgenes revealed that zygotic Lef/Tcf activity is required for the ventroposterior development of Xenopus embryos. In summary, our results suggest that endogenous Wnt/beta-catenin activity does not result in obligatory Lef/Tcf-dependent gene activation, and that the ventroposteriorizing activity of zygotic Wnt-8 signaling is mediated by Lef/Tcf proteins. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | BETA-CATENIN ; MESODERM INDUCTION ; WNT ; EXPRESSION ; ORGANIZER ; EMBRYOS ; PATHWAY ; DORSAL ; XTCF-3 ; REPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000184250600001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2395]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Geng, X,Xiao, L,Lin, FG,et al. Lef/Tcf-dependent Wnt/beta-catenin signaling during Xenopus axis specification[J]. FEBS LETTERS,2003,547(1):42010. |
| APA | Geng, X.,Xiao, L.,Lin, FG.,Hu, RY.,Wang, JH.,...&Ding, XY.(2003).Lef/Tcf-dependent Wnt/beta-catenin signaling during Xenopus axis specification.FEBS LETTERS,547(1),42010. |
| MLA | Geng, X,et al."Lef/Tcf-dependent Wnt/beta-catenin signaling during Xenopus axis specification".FEBS LETTERS 547.1(2003):42010. |
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