Substrate-induced conformational changes in Escherichia coli arginyl-tRNA synthetase observed by F-19 NMR spectroscopy
文献类型：期刊论文
| 作者 | Yao, YN; Zhang, QS; Yan, XZ; Zhu, G; Wang, ED |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2003 |
| 卷号 | 547期号:1页码:197-200 |
| 关键词 | aminoacyl-tRNA synthetase tRNA(2)(ARG) arginine ATP 4-fluorotryptophan Escherichia coli |
| 通讯作者 | Wang, ED (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | The F-19 nuclear magnetic resonance (NMR) spectra of 4-fluorotryptophan (4-F-Trp)-labeled Escherichia coli argin-yl-tRNA symbetase (ArgRS) show that there are distinct conformational changes in the catalytic core and tRNA anticodon stem and loop-binding domain of the enzyme, when arginine and tRNA(Arg) are added to the unliganded enzyme. We have assigned five fluorine resonances of 4-F-Trp residues (162, 172, 228, 349 and 446) in the spectrum of the fluorinated enzyme by site-directed mutagenesis. The local conformational changes of E. coli ArgRS induced by its substrates observed herein by F-19 NMR are similar to those of crystalline yeast homologous enzyme. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | TRYPTOPHAN RESIDUES ; BINDING ; PROTEIN ; PURIFICATION ; RECOGNITION ; RECEPTOR ; IDENTITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000184250600038 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2396]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yao, YN,Zhang, QS,Yan, XZ,et al. Substrate-induced conformational changes in Escherichia coli arginyl-tRNA synthetase observed by F-19 NMR spectroscopy[J]. FEBS LETTERS,2003,547(1):197-200. |
| APA | Yao, YN,Zhang, QS,Yan, XZ,Zhu, G,&Wang, ED.(2003).Substrate-induced conformational changes in Escherichia coli arginyl-tRNA synthetase observed by F-19 NMR spectroscopy.FEBS LETTERS,547(1),197-200. |
| MLA | Yao, YN,et al."Substrate-induced conformational changes in Escherichia coli arginyl-tRNA synthetase observed by F-19 NMR spectroscopy".FEBS LETTERS 547.1(2003):197-200. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。