An E2F site in the 5 '-promoter region contributes to serum-dependent up-regulation of the human proliferating cell nuclear antigen gene
文献类型：期刊论文
| 作者 | Li, YY; Wang, L; Lu, CD |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2003 |
| 卷号 | 544期号:1页码:112-118 |
| 关键词 | human proliferating cell nuclear antigen E2F cell cycle |
| 通讯作者 | Lu, CD (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | The synthesis of proliferating cell nuclear antigen (PCNA) is strictly regulated during the cell cycle. To investigate the contribution of the promoter region to the up-regulation of human PCNA expression at the onset of S phase, we have examined 17 putative elements with reporter assays in quiescent L-O2 cells and following serum stimulation. The E2F-like sequence 5'-TTCCCCGCAA-3' located at -84 to -75 is required for the serum-induced transactivation. In electrophoretic mobility shift assays, nuclear extracts from asynchronous L-O2 cells exhibit two binding activities toward the -75 E2F oligonucleotide, and the minor band, whose formation could be interfered with by E2F-1 antibody, represents an S phase-specific complex. This is the first demonstration of the F2F site in the human PCNA 5' promoter as a serum-responsive element. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | DIHYDROFOLATE-REDUCTASE GENE ; IN-VIVO STRUCTURE ; PCNA GENE ; S-PHASE ; TRANSCRIPTIONAL ACTIVATION ; CYCLE PROGRESSION ; GROWTH-REGULATION ; PROMOTER ; EXPRESSION ; ELEMENT |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000183393500020 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2397]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, YY,Wang, L,Lu, CD. An E2F site in the 5 '-promoter region contributes to serum-dependent up-regulation of the human proliferating cell nuclear antigen gene[J]. FEBS LETTERS,2003,544(1):112-118. |
| APA | Li, YY,Wang, L,&Lu, CD.(2003).An E2F site in the 5 '-promoter region contributes to serum-dependent up-regulation of the human proliferating cell nuclear antigen gene.FEBS LETTERS,544(1),112-118. |
| MLA | Li, YY,et al."An E2F site in the 5 '-promoter region contributes to serum-dependent up-regulation of the human proliferating cell nuclear antigen gene".FEBS LETTERS 544.1(2003):112-118. |
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