The processing of human mitochondrial leucyl-tRNA synthetase in the insect cells
文献类型：期刊论文
| 作者 | Yao, YN; Wang, L; Wu, XF; Wang, ED |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2003 |
| 卷号 | 534期号:1页码:139-142 |
| 关键词 | human mitochondrion aminoacyl-tRNA synthetase tRNA(Leu) baculovirus Aquifex aeolicus Escherichia coli |
| 通讯作者 | Wang, ED (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | A His-tagged full-length cDNA of human mitochondrial leucyl-tRNA synthetase was expressed in a baculovirus system. The N-terminal sequence of the enzyme isolated from the mitochondria of insect cells was found to be IYSATGKWT-KEYTL, indicating that the mitochondrial targeting signal peptide was cleaved between Ser39 and Ile40 after the enzyme precursor was translocated into mitochondria. The enzyme purified from mitochondria catalyzed the leucylation of Escherichia coli tRNAL(1)(Leu) (CAG) and Aquifex aeolicus tRNA(Leu) (GAG) with higher catalytic activity in the leucylation of E. coli tRNA(Leu) than that previously expressed in E. coli without the N-terminal 21 residues. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | TRANSFER-RNA-SYNTHETASE ; PURIFICATION ; SEQUENCE ; DEHYDROGENASE ; EXPRESSION ; ENZYME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000180502100024 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2399]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Yao, YN,Wang, L,Wu, XF,et al. The processing of human mitochondrial leucyl-tRNA synthetase in the insect cells[J]. FEBS LETTERS,2003,534(1):139-142. |
| APA | Yao, YN,Wang, L,Wu, XF,&Wang, ED.(2003).The processing of human mitochondrial leucyl-tRNA synthetase in the insect cells.FEBS LETTERS,534(1),139-142. |
| MLA | Yao, YN,et al."The processing of human mitochondrial leucyl-tRNA synthetase in the insect cells".FEBS LETTERS 534.1(2003):139-142. |
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