MET3 promoter: A tightly regulated promoter and its application in construction of conditional lethal strain
文献类型：期刊论文
| 作者 | Mao, XC; Hu, YF; Liang, C; Lu, CD |
| 刊名 | CURRENT MICROBIOLOGY
 |
| 出版日期 | 2002 |
| 卷号 | 45期号:1页码:37-40 |
| 通讯作者 | Lu, CD (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China., |
| 英文摘要 | The beta-galactosidase activity driven by MET3 promoter was assayed in the absence of methionine and in the presence of different concentration of methionine in medium. To compare its activity with GAL1 promoter and the data reported by Mumburg about MET25 promoter, the MET3 promoter was a weak but tightly controlled promoter. Its successful application in the construction of methionine-sensitive tri-mutant (cln1Deltacln2Deltapho85Delta) demonstrated that the MET3 promoter is a useful promoter in construction of conditional lethal strain and heterologous expression in Saccharomyces cerevisiae. |
| 学科主题 | Microbiology |
| 类目[WOS] | Microbiology |
| 关键词[WOS] | SACCHAROMYCES-CEREVISIAE ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000176025800007 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2408]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Mao, XC,Hu, YF,Liang, C,et al. MET3 promoter: A tightly regulated promoter and its application in construction of conditional lethal strain[J]. CURRENT MICROBIOLOGY,2002,45(1):37-40. |
| APA | Mao, XC,Hu, YF,Liang, C,&Lu, CD.(2002).MET3 promoter: A tightly regulated promoter and its application in construction of conditional lethal strain.CURRENT MICROBIOLOGY,45(1),37-40. |
| MLA | Mao, XC,et al."MET3 promoter: A tightly regulated promoter and its application in construction of conditional lethal strain".CURRENT MICROBIOLOGY 45.1(2002):37-40. |
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