Cloning of anti-LPS factor cDNA from Tachypleus tridentatus, expression in Bombyx mori larvae and its biological activity in vitro
文献类型：期刊论文
| 作者 | Wang, DN; Liu, JW; Yang, GZ; Zhang, WJ; Wu, XF |
| 刊名 | MOLECULAR BIOTECHNOLOGY
 |
| 出版日期 | 2002 |
| 卷号 | 21期号:1页码:42011 |
| 关键词 | Tachypleus tridentatus amebocytes anti-lipopolysaccharide (LPS) factor TALF cloning expression LPS neutralization bacteriostatic assay |
| 通讯作者 | , |
| 英文摘要 | In this article we report the cloning and expression of a cDNA encoding Tachyleus anti-lipopolysaccharide (LPS) factor, which is of interest for use as a potential inhibitor of the common core subunit of Gramnegative bacterial endotoxins. First, two degenerate primers were designed based on the sequence homology of anti-LPS factors purified from different species of horseshoe crab. The total RNA was extracted from amebocytes of Tachypleus tridentatus. The cDNA was then obtained by using the RT-PCR methods. Second, the cDNA of Tachypleus anti-LPS factor (TALF) was expressed in Bombyx mori larvae using baculovirus expression system, which showed a yield of up to 600 mg/L. Last, we determined the biological activity of the recombinant proteins by LPS neutralization assay and bacteriostatic assay in vitro. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | LIMULUS ANTILIPOPOLYSACCHARIDE FACTOR ; HORSESHOE-CRAB ; LIPOPOLYSACCHARIDE FACTOR ; ANTICOAGULANT ; ENDOTOXIN ; ACTIVATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000175065700001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2413]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, DN,Liu, JW,Yang, GZ,et al. Cloning of anti-LPS factor cDNA from Tachypleus tridentatus, expression in Bombyx mori larvae and its biological activity in vitro[J]. MOLECULAR BIOTECHNOLOGY,2002,21(1):42011. |
| APA | Wang, DN,Liu, JW,Yang, GZ,Zhang, WJ,&Wu, XF.(2002).Cloning of anti-LPS factor cDNA from Tachypleus tridentatus, expression in Bombyx mori larvae and its biological activity in vitro.MOLECULAR BIOTECHNOLOGY,21(1),42011. |
| MLA | Wang, DN,et al."Cloning of anti-LPS factor cDNA from Tachypleus tridentatus, expression in Bombyx mori larvae and its biological activity in vitro".MOLECULAR BIOTECHNOLOGY 21.1(2002):42011. |
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