Anti-HIV-1 property of trichosanthin correlates with its ribosome inactivating activity
文献类型：期刊论文
| 作者 | Wang, JH; Nie, HL; Tam, SC; Huang, H; Zheng, YT |
| 刊名 | FEBS LETTERS
 |
| 出版日期 | 2002 |
| 卷号 | 531期号:2页码:295-298 |
| 关键词 | trichosanthin ribosome inactivating protein human immunodeficiency virus type 1 antiviral activity |
| 通讯作者 | Zheng, YT (reprint author), Chinese Acad Sci, Kunming Inst Zool, Kunming 650223, Peoples R China., |
| 英文摘要 | Trichosanthin (TCS) is a type I ribosome inactivating (RI) protein possessing anti-tumor and antiviral activity, including human immunodeficiency virus (HIV). The mechanism of these actions is not entirely clear, but is generally attributed to its RI property. In order to study the relationship between the anti-HIV-1 activity of TCS and its RI activity, three TCS mutants with different RI activities were constructed by using site-directed mutagenesis. The anti-HIV-1 activities of the three mutants were tested in vitro. Results showed that two TCS mutants, namely TCSM((120-123)), TCSE160A/E189A, with the greatest decrease in RI activity, lost almost all of the anti-HIV activity and cytopathic effect. Another mutant TCSR122G, which exhibited a 160-fold decrease in RI activity, retained some anti-HIV activity. The results from this study suggested that RI activity of TCS may have significant contribution to its anti-HIV-1 property. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics; Cell Biology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 关键词[WOS] | ANTI-HIV ; PROTEIN ; CELLS ; REPLICATION ; INHIBITION ; MAP30 ; GLQ223 ; TYPE-1 ; GAP31 ; SITE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000179127900034 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2431]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, JH,Nie, HL,Tam, SC,et al. Anti-HIV-1 property of trichosanthin correlates with its ribosome inactivating activity[J]. FEBS LETTERS,2002,531(2):295-298. |
| APA | Wang, JH,Nie, HL,Tam, SC,Huang, H,&Zheng, YT.(2002).Anti-HIV-1 property of trichosanthin correlates with its ribosome inactivating activity.FEBS LETTERS,531(2),295-298. |
| MLA | Wang, JH,et al."Anti-HIV-1 property of trichosanthin correlates with its ribosome inactivating activity".FEBS LETTERS 531.2(2002):295-298. |
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