Expression and purification of Rhizobium leguminosarum NodD
文献类型：期刊论文
| 作者 | Jie, F; Feng, QL; Qiang, L; Hai, LH; Guo, FH |
| 刊名 | PROTEIN EXPRESSION AND PURIFICATION
 |
| 出版日期 | 2002 |
| 卷号 | 26期号:2页码:321-328 |
| 通讯作者 | Guo, FH (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,gfhong@sunm.shcnc.ac.cn |
| 英文摘要 | A Rhizobium expression system was constructed via introducing strong transcriptional elements to an IncP broad host range plasmid pKT230. Using this system, the nodD gene of Rhizobium leguminosarum biovar viciae was overexpressed in its own in vivo environment. Western blot showed that the NodD yield of the newly constructed expression vector pKNDT was much higher than that of a reported vector pIJ1518. Based on the result from quantitative gel retardation assay, the specific DNA-binding activity of NodD was estimated to increase by about 80-fold. Purification of NodD was performed by the sequential steps: polyethyleneimine treatment, ammonium sulfate fractional precipitation, and cellulose phosphate P11 chromatography. About 26 mg NodD with a purity about 90 % was obtained from 14g wet weight cell pellet within 2 days. (C) 2002 Elsevier Science (USA). All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 关键词[WOS] | BROAD HOST RANGE ; NODULATION ; SYSTEM ; GENES ; SITES ; DNA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000179224500017 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2432]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Jie, F,Feng, QL,Qiang, L,et al. Expression and purification of Rhizobium leguminosarum NodD[J]. PROTEIN EXPRESSION AND PURIFICATION,2002,26(2):321-328. |
| APA | Jie, F,Feng, QL,Qiang, L,Hai, LH,&Guo, FH.(2002).Expression and purification of Rhizobium leguminosarum NodD.PROTEIN EXPRESSION AND PURIFICATION,26(2),321-328. |
| MLA | Jie, F,et al."Expression and purification of Rhizobium leguminosarum NodD".PROTEIN EXPRESSION AND PURIFICATION 26.2(2002):321-328. |
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