Structural studies of an eukaryotic cambialistic superoxide dismutase purified from the mature seeds of camphor tree
文献类型：期刊论文
| 作者 | Chen, HY; Hu, RG; Wang, BZ; Chen, WF; Liu, WY; Schroder, W; Frank, P; Ulbrich, N |
| 刊名 | ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2002 |
| 卷号 | 404期号:2页码:218-226 |
| 关键词 | expression Fe-superoxide dismutase primary structure reconstruction of metal cofactors refolding RT-PCR |
| 通讯作者 | Liu, WY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | An iron-superoxide dismutase (SOD) was purified and characterized from the mature seeds of camphor tree (Cinnamomum camphora). The ultraviolet and visible absorption spectra of camphor Fe-SOD showed patterns typical of cambialistic Fe-SODs. The inductively coupled plasma assay indicated that there was 0.5-1 atom of Fe2+ per camphor Fe-SOD subunit. The cDNA of camphor Fe-SOD, including the coding region and the 3' noncoding region, was obtained by reverse transcription polymerase chain reaction using the total RNA from immature seeds of C camphora as template and then sequenced. The complete amino acid sequence of camphor Fe-SOD was deduced from the cDNA sequence. The correctness of the amino acid sequence was confirmed by directly sequencing five peptide fragments of the enzyme. The molecular mass calculated for the camphor Fe-SOD subunit from its 204 amino acid residues was 22,930.6 Da, The cDNA of camphor Fe-SOD was cloned into the expression vector PMFT7-5 and then expressed in Escherichia coli strain BL21. The reconstructed Fe- or Mn-SOD was purified to homogeneity through column chromatography. Activity of the Fe- or Mn-SOD was found to be almost equal to that of natural camphor Fe-SOD, which is the first cambialistic SOD isolated from eukaryotic cells. (C) 2002 Elsevier Science (USA). All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | AMINO-ACID-SEQUENCE ; ESCHERICHIA-COLI ; PROTEIN MOIETY ; MANGANESE ; IRON ; PEROXISOMES ; PLANTS ; PURIFICATION ; NUCLEOTIDE ; ENZYME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000177515400005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2435]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Chen, HY,Hu, RG,Wang, BZ,et al. Structural studies of an eukaryotic cambialistic superoxide dismutase purified from the mature seeds of camphor tree[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,2002,404(2):218-226. |
| APA | Chen, HY.,Hu, RG.,Wang, BZ.,Chen, WF.,Liu, WY.,...&Ulbrich, N.(2002).Structural studies of an eukaryotic cambialistic superoxide dismutase purified from the mature seeds of camphor tree.ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,404(2),218-226. |
| MLA | Chen, HY,et al."Structural studies of an eukaryotic cambialistic superoxide dismutase purified from the mature seeds of camphor tree".ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 404.2(2002):218-226. |
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