Study of the structural environment of DsbA protein by labeling of tryptophan analogs
文献类型：期刊论文
| 作者 | Li, Q; Hu, HY; Sheng, WY; Zhao, XY; Xu, GJ |
| 刊名 | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2002 |
| 卷号 | 29期号:2页码:319-322 |
| 关键词 | biosynthetic labeling fluorescence F-19-NMR DsbA protein structural environment |
| 通讯作者 | , |
| 英文摘要 | Tryptophan analogs were biosynthetically incorporated into the tryptophan sites of DsbA protein to investigate the spectroscopic characteristics, local environments of tryptophans. This technique by using tryptophan-analog labeling may be of potential application to studying structure-function relationships and protein-protein interactions. 5-hydroxytryptophan labeled DsbA protein presents a fluorescence excitation wavelength at 315 rim and an emission near 340 rim. The chemical shifts of Trp76 and Trp126 in 5-fluorotrytophan labeled DsbA were identified in F-19-NMR spectra, and change of the chemical shift of Trp76. p can reflect the conformational change of DsbA protein during redox reaction. The future work is to use specific fluorescence and F-19-NMR of labeled DsbA protein to study its redox properties and interaction with substrates. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000175332200031 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2441]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Li, Q,Hu, HY,Sheng, WY,et al. Study of the structural environment of DsbA protein by labeling of tryptophan analogs[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2002,29(2):319-322. |
| APA | Li, Q,Hu, HY,Sheng, WY,Zhao, XY,&Xu, GJ.(2002).Study of the structural environment of DsbA protein by labeling of tryptophan analogs.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,29(2),319-322. |
| MLA | Li, Q,et al."Study of the structural environment of DsbA protein by labeling of tryptophan analogs".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 29.2(2002):319-322. |
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