Nonspecific deadenylation on sarcin/ricin domain RNA catalyzed by gelonin under acidic conditions
文献类型：期刊论文
| 作者 | He, WJ; Tang, SA; Liu, WY; Stirpe, F |
| 刊名 | ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
 |
| 出版日期 | 2002 |
| 卷号 | 399期号:2页码:181-187 |
| 关键词 | gelonin ribosome-inactivating protein (RIP) RNA N-glycosidase sarcin/ricin domain RNA (SRD RNA) site specificity nonspecific deadenlyation |
| 通讯作者 | Liu, WY (reprint author), Chinese Acad Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Gelonin is a single-chain ribosome-inactivating protein that can hydrolyze the glycosidic bond of a highly conserved adenosine residue in the sarcin/ricin domain (SRD) of the largest RNA in ribosome and thus irreversibly inhibit protein synthesis. Recently, the specificity in substrate recognition was challenged by the fact that gelonin could remove adenines from some other oligoribonucleotide substrates. However, the site specificity of gelonin to deadenylate various substrates were unknown. Hereby, the effect of pH values upon site specificity of the deadenylation activity of gelonin was studied using the synthetic oligoribonucleotide (named SRD RNA) that mimicked the ribosomal. SRD. Interestingly, gelonin gradually acquired the ability to nonspecifically remove adenines from SRD RNA when pH values changed from neutral to acidic conditions. Another two SRD RNA mutants, either with the conserved adenosine deleted or with the tetraloop converted, showed very similar cleavage style to wild-type SRD RNA, underscoring the important role of pH value in site specificity of recognition by gelonin. Furthermore, the RNA N-glycosidase activity of gelonin was also enhanced with the decreasing of pH values. In addition, no obvious change was observed in the molecular conformation of gelonin at various pH values. Taken together, our data implied that the protonation of adenosines in SRD RNA was potentially an important factor for the nonspecific deadenlyation by gelonin. (C) 2002 Elsevier Science (USA). |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | RIBOSOME-INACTIVATING PROTEINS ; POLYNUCLEOTIDE-ADENOSINE GLYCOSIDASE ; A-CHAIN RECOGNITION ; ELONGATION-FACTORS ; IDENTITY ELEMENTS ; ALPHA-SARCIN ; DNA ; DEGRADATION ; MECHANISM ; PLANTS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000174586500008 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2445]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | He, WJ,Tang, SA,Liu, WY,et al. Nonspecific deadenylation on sarcin/ricin domain RNA catalyzed by gelonin under acidic conditions[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,2002,399(2):181-187. |
| APA | He, WJ,Tang, SA,Liu, WY,&Stirpe, F.(2002).Nonspecific deadenylation on sarcin/ricin domain RNA catalyzed by gelonin under acidic conditions.ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,399(2),181-187. |
| MLA | He, WJ,et al."Nonspecific deadenylation on sarcin/ricin domain RNA catalyzed by gelonin under acidic conditions".ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 399.2(2002):181-187. |
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