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Phosphorylation of YLR190w by PAP1-PHO85 kinase complex

文献类型：期刊论文

作者Shi, XZ; Ao, SZ
刊名ACTA BIOCHIMICA ET BIOPHYSICA SINICA
出版日期2002
卷号34期号:2页码:187-192
关键词PAP1 PHO85 phosphorylation yeast two-hybrid system YLR190w
通讯作者,
英文摘要A yeast two-hybrid screening by using PAPI was performed to identify targets for PAP1-PHO85 cyclin-CDK complex. N-terminal fragment of protein YLR190w, a yeast gene encoding a 491 amino acids peptide, was identified, and its coding region was amplified by PCR. The interaction of PAP1 and YLR190w was confirmed by both two-hybrid assay and GST pull-down assay in vitro. The PAP1-PHO85 kinase complex obtained from the immunoprecipitates could phosphorylate GST-YLR190w expressed in E. coli, and the phosphorylation of YLR190w was affected by the phosphate concentration, and the phosphorylation sites of YLR190w were Ser/Thr-Pro motif, as revealed by protein mutation assay. In another library screen, YAF9, a yeast homolog of human AF9, was isolated using the two-hybrid system with YLR190w as the bait. It was revealed that interaction of YLR190w and YAF9 was affected by phosphate concentration. When all Ser/Thr in Ser/Thr-Pro motif were mutated to Ala, the interaction of YLR190w (mutant) and YAF9 was weakened, and the effect of phosphate concentration was impaired. Ylr190w was not involved in the PHO system by the acid phosphatase activity assay. Deletion of Ylr190w was constructed by homologous recombination and the doubling time of Ylr190w mutant strain was longer than that of wild type.
学科主题Biochemistry & Molecular Biology; Biophysics
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]CYCLIN-DEPENDENT KINASE ; GLUTATHIONE-S-TRANSFERASE ; SACCHAROMYCES-CEREVISIAE ; BUDDING YEAST ; CELL-CYCLE ; PROTEIN-KINASE ; CDK INHIBITOR ; PHO85 ; PHO80-PHO85 ; EXPRESSION
收录类别SCI
语种中文
WOS记录号WOS:000174549400012
版本出版稿
源URL[http://202.127.25.143/handle/331003/2448]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714		 Shi, XZ,Ao, SZ. Phosphorylation of YLR190w by PAP1-PHO85 kinase complex[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(2):187-192.
APA Shi, XZ,&Ao, SZ.(2002).Phosphorylation of YLR190w by PAP1-PHO85 kinase complex.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(2),187-192.
MLA Shi, XZ,et al."Phosphorylation of YLR190w by PAP1-PHO85 kinase complex".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.2(2002):187-192.

入库方式： OAI收割

来源：上海生物化学与细胞生物学研究所

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