Antiviral activity of a hammerhead ribozyme against HBV in HepG2.2-15 cells
文献类型：期刊论文
| 作者 | Feng, Y; Kong, YY; Wang, Y; Qi, GR |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2002 |
| 卷号 | 34期号:2页码:204-208 |
| 关键词 | hammerhead ribozyme HBV HepG2.2.15 cells |
| 通讯作者 | , |
| 英文摘要 | The carboxy terminal arginine-rich domain of core protein is highly conserved among HBV subtypes, and plays important roles in the packaging of pregenomic RNA and viral replication. Therefore, the 3' highly conserved region of HBV C gene is an attractive target for antiviral therapy mediated by ribozymes. A hammerhead ribozyme RzC, targeting the above site, was designed; meanwhile, as controls, the disabled form of RzC, dRzC, was also designed. In order to investigate its antiviral effects in cultured cells, in vitro-transcribed ribozyme RzC, dRzC and ribozyme expression vectors pCRzC, pCdRzC were delivered, respectively, into HepG2.2.15 cells ( clonal cells derived from HepG2 cells that contain integrated HBVayw DNA). The preliminary results demonstrated that in vitro-transcribed ribozyme RzC had weak inhibition on HBV replication, possibly due to its quick degradation by RNases in cells, while the endogenously expressed ribozyme RzC showed significant inhibitory effect on HBV replication. In conclusion, the results suggested the possibility of the hammerhead ribozyme RzC to be used for the gene therapy of HBV infection. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | HEPATITIS-B VIRUS ; NUCLEAR-LOCALIZATION ; CORE PROTEIN ; IN-VITRO ; REPLICATION ; INHIBITION ; INFECTION ; PHOSPHORYLATION ; INVITRO ; THERAPY |
| 收录类别 | SCI |
| 语种 | 中文 |
| WOS记录号 | WOS:000174549400015 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2449]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Feng, Y,Kong, YY,Wang, Y,et al. Antiviral activity of a hammerhead ribozyme against HBV in HepG2.2-15 cells[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(2):204-208. |
| APA | Feng, Y,Kong, YY,Wang, Y,&Qi, GR.(2002).Antiviral activity of a hammerhead ribozyme against HBV in HepG2.2-15 cells.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(2),204-208. |
| MLA | Feng, Y,et al."Antiviral activity of a hammerhead ribozyme against HBV in HepG2.2-15 cells".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.2(2002):204-208. |
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