Up-regulation of cystatin C expression in the murine hippocampus following perforant path transections
文献类型：期刊论文
| 作者 | Ying, GX; Huang, C; Jiang, ZH; Liu, X; Jing, NH; Zhou, CF |
| 刊名 | NEUROSCIENCE
 |
| 出版日期 | 2002 |
| 卷号 | 112期号:2页码:289-298 |
| 关键词 | deafferentation protease inhibitor astrocyte in situ hybridization immunohistochemistry |
| 通讯作者 | Zhou, CF (reprint author), Chinese Acad Sci, Shanghai Inst Physiol, Key Lab Neurobiol, Shanghai Inst Biol Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China., |
| 英文摘要 | Cystatins are endogenous cysteine protease inhibitors that modulate the turnover of intracellular and extracellular proteins. These inhibitors are strongly implicated in a variety of pathological processes such as tumor metastasis and many degenerating CNS disorders. Here we report the expression of cystatin C, a major cysteine protease inhibitor of mammalian animals, in the murine hippocampus at 3, 7, 15 and 30 days following perforant path transections. Northern blot analysis showed that cystatin C transcripts were up-regulated in a transient manner with a significant increase at 7 and 15 days post-lesion (219% and 185% of control, respectively) in the rat hippocampus after entorhinal deafferentation. In situ hybridization and immunohistochemistry confirmed the time-dependent up-regulation of both cystatin C mRNA and protein expressions in a mouse model which initiated at 3 days post-lesion, reached maximal levels 7-15 days post-lesion, and remained slightly elevated by day 30 post-lesion. The modulation of cystatin C expression was observed to occur specifically in the entorhinally denervated zones: the stratum lacunosum-moleculare of the hippocampus and the outer molecular layer of the dentate gyrus. Double labeling by either a combination of in situ hybridization for cystatin C with immumohistochemistry for glial fibrillary acidic protein or double immunofluorescence staining for both proteins in mouse hippocampus at 7 and 15 days post-lesion revealed that most cystatin C-expressing cells are astrocytes. From these results we suggest that the spatiotemporal up-regulation of cystatin C in the hippocampus is induced by entorhinal deafferentation and that cystatin C may be involved in the astroglia-mediated neural plasticity events in the hippocampus following perforant path transections. (C) 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved. |
| 学科主题 | Neurosciences & Neurology |
| 类目[WOS] | Neurosciences |
| 关键词[WOS] | CYSTEINE-PROTEINASE-INHIBITOR ; ENTORHINAL CORTEX LESION ; DEAFFERENTED DENTATE GYRUS ; FIBRILLARY ACIDIC PROTEIN ; ADULT-RAT ; MESSENGER-RNA ; CATHEPSIN-B ; INDUCED SYNAPTOGENESIS ; REACTIVE ASTROCYTES ; NEURITE OUTGROWTH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000176525700005 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2453]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Ying, GX,Huang, C,Jiang, ZH,et al. Up-regulation of cystatin C expression in the murine hippocampus following perforant path transections[J]. NEUROSCIENCE,2002,112(2):289-298. |
| APA | Ying, GX,Huang, C,Jiang, ZH,Liu, X,Jing, NH,&Zhou, CF.(2002).Up-regulation of cystatin C expression in the murine hippocampus following perforant path transections.NEUROSCIENCE,112(2),289-298. |
| MLA | Ying, GX,et al."Up-regulation of cystatin C expression in the murine hippocampus following perforant path transections".NEUROSCIENCE 112.2(2002):289-298. |
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