Heat shock response inhibits IL-18 expression through the JNK pathway in murine peritoneal macrophages
文献类型：期刊论文
| 作者 | Wang, Y; Li, CL; Wang, XY; Zhang, JS; Chang, ZL |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2002 |
| 卷号 | 296期号:3页码:742-748 |
| 关键词 | heat shock response IL-18 JNK AP-1 macrophages |
| 通讯作者 | Chang, ZL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China., |
| 英文摘要 | Heat shock response has been implicated in cytoprotective effects from cellular damage and in the regulation of cytokine expression. We report the effect of heat shock on LPS-induced expression of IL-18, an important cytokine that has diverse immune regulatory effects on T cells, B cells, NK cells, and nonimmune cells. The augmentation of LPS-induced IL-18 mRNA and protein was significantly suppressed in murine peritoneal macrophages after 43 degreesC heat shock treatment. In addition, the JNK MAPK inhibitor SP600125 inhibited IL-18 mRNA transcription in a dose-dependent manner. To examine the possibility that the inhibition of IL-18 may be mediated through the inactivation of JNK, the activity of JNK was measured by using Western blot and kinase assays. Our data show that heat shock response decreased LPS-induced phosphorylation of JNK and its downstream substrate c-Jun. AP-1, a transcriptional factor composed of c-Jun, could regulate the expression of IL-18. Also, its DNA-binding activity was reduced by the heat shock response. These findings suggest that treatment of heat stress results in inhibition of IL-18 production in macrophages mainly through the JNK/AP-1 signaling pathway. (C) 2002 Elsevier Science (USA). All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | NF-KAPPA-B ; GENE-EXPRESSION ; IFN-GAMMA ; INTERLEUKIN-12 ; ACTIVATION ; PROTEINS ; KINASES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000177747800036 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2457]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Y,Li, CL,Wang, XY,et al. Heat shock response inhibits IL-18 expression through the JNK pathway in murine peritoneal macrophages[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2002,296(3):742-748. |
| APA | Wang, Y,Li, CL,Wang, XY,Zhang, JS,&Chang, ZL.(2002).Heat shock response inhibits IL-18 expression through the JNK pathway in murine peritoneal macrophages.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,296(3),742-748. |
| MLA | Wang, Y,et al."Heat shock response inhibits IL-18 expression through the JNK pathway in murine peritoneal macrophages".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 296.3(2002):742-748. |
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