Metabolic responses induced by thrombin in human umbilical vein endothelial cells
文献类型：期刊论文
| 作者 | Fan, Y; Wu, DZ; Gong, YQ; Xu, R; Hu, ZB |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2002 |
| 卷号 | 293期号:3页码:979-985 |
| 关键词 | thrombin acidification rate MAP kinase PKC calcium human umbilical vein endothelial cells |
| 通讯作者 | Wu, DZ (reprint author), Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, Shanghai 200032, Peoples R China., |
| 英文摘要 | Metabolic responses induced by thrombin in human umbilical vein endothelial cells (HUVECs) were investigated by using the cytosensor technique. Thrombin increased the extracellular acidification rate of endothelial cells, measured as an index of metabolic activity with a cytosensor microphysiometer, in a concentration-dependent fashion with an EC50 of 1.27 +/- 0.59 IU/ml, which was abolished by the MAP kinase inhibitor PD98059. When intracellular Ca2+ was chelated or PKC was inactivated, PD98059 failed to abolish the thrombin-induced acidification rate response in HUVECs. In addition, the tyrosine kinase inhibitor genistein, PKC inhibitor calphostin C, and Na+/H+ exchanger antagonist MIA also partly inhibited thrombin-induced acidification rate responses. It is suggested that thrombin stimulated rapid metabolic responses via MAP kinase in HUVECs, which are calcium- and PKC-dependent. (C) 2002 Elsevier Science (USA). All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | PROTEIN-KINASE-C ; NA+/H+ EXCHANGER ; IN-VITRO ; ACTIVATION ; PATHWAYS ; CULTURE ; PROLIFERATION ; ANGIOGENESIS ; STIMULATION ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000175640800016 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/2461]  |
| 专题 | 上海生化细胞研究所_上海生科院生化细胞研究所 |
| 推荐引用方式 GB/T 7714 | Fan, Y,Wu, DZ,Gong, YQ,et al. Metabolic responses induced by thrombin in human umbilical vein endothelial cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2002,293(3):979-985. |
| APA | Fan, Y,Wu, DZ,Gong, YQ,Xu, R,&Hu, ZB.(2002).Metabolic responses induced by thrombin in human umbilical vein endothelial cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,293(3),979-985. |
| MLA | Fan, Y,et al."Metabolic responses induced by thrombin in human umbilical vein endothelial cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 293.3(2002):979-985. |
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