中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Metabolic responses induced by thrombin in human umbilical vein endothelial cells

文献类型:期刊论文

作者Fan, Y; Wu, DZ; Gong, YQ; Xu, R; Hu, ZB
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
出版日期2002
卷号293期号:3页码:979-985
关键词thrombin acidification rate MAP kinase PKC calcium human umbilical vein endothelial cells
通讯作者Wu, DZ (reprint author), Shanghai Univ Tradit Chinese Med, Inst Chinese Mat Med, Shanghai 200032, Peoples R China.,
英文摘要Metabolic responses induced by thrombin in human umbilical vein endothelial cells (HUVECs) were investigated by using the cytosensor technique. Thrombin increased the extracellular acidification rate of endothelial cells, measured as an index of metabolic activity with a cytosensor microphysiometer, in a concentration-dependent fashion with an EC50 of 1.27 +/- 0.59 IU/ml, which was abolished by the MAP kinase inhibitor PD98059. When intracellular Ca2+ was chelated or PKC was inactivated, PD98059 failed to abolish the thrombin-induced acidification rate response in HUVECs. In addition, the tyrosine kinase inhibitor genistein, PKC inhibitor calphostin C, and Na+/H+ exchanger antagonist MIA also partly inhibited thrombin-induced acidification rate responses. It is suggested that thrombin stimulated rapid metabolic responses via MAP kinase in HUVECs, which are calcium- and PKC-dependent. (C) 2002 Elsevier Science (USA). All rights reserved.
学科主题Biochemistry & Molecular Biology ; Biophysics
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]PROTEIN-KINASE-C ; NA+/H+ EXCHANGER ; IN-VITRO ; ACTIVATION ; PATHWAYS ; CULTURE ; PROLIFERATION ; ANGIOGENESIS ; STIMULATION ; EXPRESSION
收录类别SCI
语种英语
WOS记录号WOS:000175640800016
版本出版稿
源URL[http://202.127.25.143/handle/331003/2461]  
专题上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式
GB/T 7714
Fan, Y,Wu, DZ,Gong, YQ,et al. Metabolic responses induced by thrombin in human umbilical vein endothelial cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2002,293(3):979-985.
APA Fan, Y,Wu, DZ,Gong, YQ,Xu, R,&Hu, ZB.(2002).Metabolic responses induced by thrombin in human umbilical vein endothelial cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,293(3),979-985.
MLA Fan, Y,et al."Metabolic responses induced by thrombin in human umbilical vein endothelial cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 293.3(2002):979-985.

入库方式: OAI收割

来源:上海生物化学与细胞生物学研究所

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